Lipidomics Core

脂质组学核心

• 批准号: 8308980
• 负责人: ALICJA BIELAWSKA
• 金额: $ 19.24万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2012-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8308980
• 关键词: 1,2-diacylglycerol; Aging; Anabolism; Apoptosis; Apoptotic; Area; Attention; Binding; Biocompatible Materials; Biological; Biology; Cancer Biology; Cell Aging; Cell Death; Cell physiology; Cells; Cellular biology; Ceramides; Chemistry; Complex; Coupled; Culture Media; Data Analyses; Degenerative Disorder; Development; Diagnostic; Diglycerides; Disease; Educational workshop; Eicosanoids; Endothelial Cells; Environment; Enzyme Inhibitor Drugs; Enzyme Inhibitors; Enzymes; Experimental Designs; Functional disorder; Generations; Growth; High Pressure Liquid Chromatography; Human Resources; In Vitro; Inflammation; International; Label; Link; Lipid Chemistry; Lipids; Liquid substance; Malignant Neoplasms; Mammals; Measurement; Measures; Mediating; Membrane; Mentors; Metabolic Pathway; Metabolism; Methodology; Methods; Molecular; Molecular Analysis; Molecular Probes; Monitor; Neoplasm Metastasis; Organelles; Participant; Pathway interactions; Phosphatidylinositol Phosphates; Plasma; Preparation; Procedures; Process; Program Research Project Grants; Radioactive; Radiolabeled; Reagent; Regulation; Research; Research Personnel; Resources; Role; Serum; Services; Signal Transduction; Site; Sphingolipids; Sphingomyelins; Sphingosine; Sphingosine-1-Phosphate Receptor; Stereoisomer; Technology; Therapeutic; Tissues; Training; Vertebral column; Work; Yeasts; analog; angiogenesis; arachidonate; base; biological adaptation to stress; cancer therapy; cell behavior; cell growth; cell growth regulation; cell motility; ceramide 1-phosphate; design; dihydroceramide; in vitro activity; in vivo; inhibitor/antagonist; inorganic phosphate; insight; interest; lipid mediator; lipid metabolism; metabolic abnormality assessment; novel; programs; radiotracer; receptor; research study; response; sphingosine 1-phosphate; success; tandem mass spectrometry; tool; tumor growth; tumor progression; vascular inflammation; water solubility

The diversity of bioactive lipids and their interconnected metabolism provide a network of pathways regulating intra- and inter-cellular signaling and function. Dysfunctions in these pathways contribute to the pathobiology of cancer progression and metastasis. These considerations have necessitated the development of lipid chemistry and analysis. The Lipidomics Core was created based on unique expertise of the key personnel in lipid chemistry, analysis, and metabolism. It has evolved into a key Core for the program project grant (PPG) entitled Sphingolipids in Cancer Biology and Therapy, and was used by all participating investigators. Furthermore, this Core has developed into an institutional, national, and international resource in the emerging fields of lipidomics and pathobiology. Core services include: 1) assisting PPG investigators in experimental design, selection of lipids of interest, and interpretation of analytical results; 2) providing qualitative and quantitative analysis of lipid components from different biological materials (cells, tissue, and biological fluids), primarily employing high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) technology¿the Core currently provides quantitative analysis of more than 100 distinct lipid molecular species; and 3) providing synthetic molecular tools to study lipid metabolism (e.g., functionalized and fluorescent ce ram ides, site-specific radioactive sphingolipids, 17C-sphingolipids) and diversified synthetic lipids and analogs for cellular, in vitro, and in vivo studies (e.g., organelle-targeted sphingolipids and organelle-targeted inhibitors of sphingolipid metabolizing enzymes). The Lipidomics Core has been instrumental to the success of the PPG investigators by providing them with unique capabilities and critical results that should continue to contribute for the successful progress of this PPG.

生物活性脂质的多样性及其相互关联的新陈代谢提供了一个调节途径的网络。 细胞内和细胞间的信号和功能。这些通路的功能障碍导致了病理生物学 癌症的进展和转移。这些考虑使得脂质的发展成为必要。 化学和分析。脂肪组学核心是基于以下关键人员的独特专业知识创建的 脂肪化学、分析和新陈代谢。它已发展成为计划项目拨款(PPG)的关键核心 题为“神经鞘脂在癌症生物学和治疗中”，并被所有参与的研究人员使用。 此外，这一核心已经发展成为机构、国家和国际资源 脂类组学和病理生物学的新兴领域。核心服务包括：1)协助PPG调查人员 实验设计、感兴趣的脂类的选择和分析结果的解释；2)提供 不同生物材料(细胞、组织和组织)中脂质成分的定性和定量分析 生物流体)，主要使用高效液相色谱-串联质谱仪 (HPLC-MS/MS)技术？Core目前为100多种不同的脂肪提供定量分析 分子物种；以及3)提供合成分子工具来研究脂类代谢(例如，功能化 和荧光标记物、特定部位放射性神经鞘糖脂、17C-神经鞘糖脂)和多样化 用于细胞、体外和体内研究的合成脂类和类似物(例如，细胞器靶向鞘磷脂和 神经鞘脂代谢酶的细胞器靶向抑制物)。脂肪组学的核心一直是 通过为PPG调查人员提供独特的能力和关键的 这些成果应继续为这一项目小组的成功进展作出贡献。