SC COBRE: LIPIDOMICS CORE

SC COBRE：脂质组学核心

• 批准号: 8360378
• 负责人: ALICJA BIELAWSKA
• 金额: $ 36.14万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-07-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360378
• 关键词: Biological; Biology; Cells; Centers of Research Excellence; Ceramides; Chemistry; Data; Dihydrosphingosine; Experimental Designs; Funding; Galactosylceramides; Glucosylceramides; Glycerol; Grant; High Pressure Liquid Chromatography; In Vitro; Lactosylceramides; Lipids; Metabolism; Molecular; Molecular Probes; National Center for Research Resources; Principal Investigator; Radioactive; Research; Research Infrastructure; Research Personnel; Resources; Sampling; Serum; Side; Source; Sphingolipids; Sphingomyelins; Sphingosine; Stereoisomer; Techniques; Tissues; United States National Institutes of Health; Vertebral column; analog; base; cost; dihydroceramide; in vivo; inorganic phosphate; metabolomics; operation; phytosphingosine; tandem mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Lipidomics Core is in full operation and is providing the following support to COBRE project investigators: (i) expertise in sphingolipid chemistry, analysis and biology, (ii) assistance with experimental design and interpretation of analytical data, (iii) qualitative and quantitative analysis of key sphingolipids from biological samples: cells, tissue, serum and media. Analytical approach is based on High Performance Liquid Chromatography-Tandem Mass spectrometry (LC-MS/MS) technique. Currently, core provides metabolomic profiling of ~ 100 different lipid species including: sphingosine, dihydrosphingosine, phytosphingosine and their phosphates, ceramides, alpha-hydroxy-ceramides, dihydroceramides, phytoceramides, alpha-hydroxy-phytoceramides and their phosphates, sphingomyelin, hexosyl-ceramide, lactosyl ceramide and diacyl-glycerol (DAG's) components. Additionally, a separate analysis of glucosylceramide and galactosylceramide molecular species by SCF-MS/MS approach is also provided. (iv) homogenic synthetic sphingolipids and their analogs for use in cellular, in vitro and in vivo studies. Currently, core provides stereoisomers of modified sphingoid bases, ceramides and their phosphates, sphingomyelin, modulators of sphingolipid metabolism and advanced molecular probes: functionalized, fluorescent, side-specific radioactive sphingolipids and 17C-backbone sphingolipids. Additionally, analytical approach to study sphingolipid metabolism and enzymatic activities (using sphingolipids composed of 17C-backbone and MS analysis) is also available for the COBRE investigators. Starting from July, 2010 up to April 06, 2011, the analytical subCore performed 2612 analyses for the current COBRE projects (analytical limit established for each project was estimated as 300 analyses/year for each project) and 477 analyses for the former COBRE graduates: Dr. Argraves (87), Dr. Hammad (66), Dr. Hama (44) and Dr. Gudz (280).

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 脂质组学核心已全面投入运行，并为 COBRE 项目研究人员提供以下支持：(i) 鞘脂化学、分析和生物学方面的专业知识，(ii) 协助实验设计和分析数据解释，(iii) 对生物样品（细胞、组织、血清和培养基）中的关键鞘脂进行定性和定量分析。分析方法基于高效液相色谱-串联质谱 (LC-MS/MS) 技术。目前，Core 提供约 100 种不同脂质种类的代谢组学分析，包括：鞘氨醇、二氢鞘氨醇、植物鞘氨醇及其磷酸盐、神经酰胺、α-羟基神经酰胺、二氢神经酰胺、植物神经酰胺、α-羟基植物神经酰胺及其磷酸盐、鞘磷脂、 己糖基神经酰胺、乳糖基神经酰胺和二酰基甘油 (DAG) 成分。此外，还提供了通过 SCF-MS/MS 方法对葡萄糖神经酰胺和半乳糖神经酰胺分子种类的单独分析。 (iv) 用于细胞、体外和体内研究的均质合成鞘脂及其类似物。目前，Core提供改性鞘氨醇碱的立体异构体、神经酰胺及其磷酸盐、鞘磷脂、鞘脂代谢调节剂和先进的分子探针：功能化、荧光、侧特异性放射性鞘脂和17C-骨架鞘脂。 此外，COBRE 研究人员还可以使用分析方法来研究鞘脂代谢和酶活性（使用由 17C 主链组成的鞘脂和 MS 分析）。 从 2010 年 7 月到 2011 年 4 月 6 日，分析子核心对当前 COBRE 项目进行了 2612 次分析（为每个项目确定的分析限制估计为每个项目每年 300 次分析），并为前 COBRE 毕业生进行了 477 次分析：Argraves 博士 (87)、Hammad 博士 (66)、Hama 博士 (44) 和 Gudz 博士（280）。