FASTER IDENTIFICATION OF EPIDEMIC BACTERIAL PATHOGENS ON THE US-MEXICAN BORDER

更快地识别美墨边境的流行性细菌病原体

• 批准号: 8361755
• 负责人: JOHN E GUSTAFSON
• 金额: $ 1.12万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361755
• 关键词: Antibiotic Resistance; Area; Bacterial Typing; Development; Disease Outbreaks; Effectiveness; Epidemic; Evolution; Faculty; Flow Cytometry; Foundations; Funding; Gold; Grant; Infection; Laboratories; Lead; Libraries; Mexican; Molecular; Molecular Epidemiology; Morbidity - disease rate; National Center for Research Resources; Natural History; Patients; Pilot Projects; Principal Investigator; Research; Research Infrastructure; Research Personnel; Resources; Sorting - Cell Movement; Source; Speed; Techniques; Technology; Testing; Time; United States National Institutes of Health; base; cost; methicillin resistant Staphylococcus aureus; mortality; novel; pathogen; tool; ward

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Multiple-antibiotic-resistant, methicillin-resistant Staphylococcus aureus (MRSA) presents a major threat to infected patients. The majority of infections caused by MRSA on a global scale are caused by certain clones, often referred to as "epidemic MRSA". The identification of patients infected with epidemic MRSA clones and application of stringent barrier techniques and contact isolation to the associated wards and areas, can lead to a halt in nosocomial MRSA outbreaks and reduce overall morbidity and mortality. In this pilot project we intend to: 1. Determine the molecular identity of MRSA clones in the Las Cruces region; 2. Determine the effectiveness of high-sensitivity flow cytometry (FCM) in determining MRSA strain relatedness in a real world setting, in comparison to established MRSA molecular typing techniques; 3. Produce a natural history library of MRSA strains from the Paso Del Norte region for exchange with researchers around the world and further examination of novel MRSA clone introductions and/or local geographical evolution; and 4. Develop the scientific foundation for the development of a bacterial pathogen-typing center on the US-Mexican border. We hypothesis that a unique flow cytometry (FCM)-based technique developed at LANL will comparably differentiate MRSA strains as well as two gold standard molecular typing techniques, and in less time. Proof that the FCM technique is an effective tool for determining MRSA strain molecular identity and relatedness, will help legitimize this technology and allow FCM to increase the speed at which epidemic MRSA clones and other pathogenic bacterial clones are identified. Furthermore, establishment of a strong molecular epidemiology/bacterial typing unit in our region could theoretically be called into duty to identify outbreaks of potential bacterial bioweapons and used to track the source of such infections in a relatively short time. In addition, this pilot study will establish a firm relationship between the laboratory of Babetta Marrone at LANL which is developing cutting edge bacterial typing/identification technology and NMSU faculty where some of these techniques can be tested in a real world setting.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 多重抗生素耐药、耐甲氧西林金黄色葡萄球菌 (MRSA) 对感染患者构成重大威胁。全球范围内MRSA引起的大多数感染都是由某些克隆引起的，通常被称为“流行性MRSA”。识别感染流行性 MRSA 克隆的患者，并对相关病房和区域应用严格的屏障技术和接触隔离，可以阻止医院内 MRSA 的爆发，并降低总体发病率和死亡率。在这个试点项目中，我们打算： 1. 确定拉斯克鲁塞斯地区MRSA克隆的分子身份； 2. 与已建立的 MRSA 分子分型技术相比，确定高灵敏度流式细胞术 (FCM) 在确定现实世界中 MRSA 菌株相关性方面的有效性； 3. 建立北帕索德尔北地区 MRSA 菌株的自然历史文库，以便与世界各地的研究人员进行交流，并进一步检查新的 MRSA 克隆引入和/或当地地理进化；和 4. 为美墨边境细菌病原体分型中心的发展奠定科学基础。 我们假设 LANL 开发的基于流式细胞术 (FCM) 的独特技术将能够在更短的时间内与两种金标准分子分型技术一样区分 MRSA 菌株。证明 FCM 技术是确定 MRSA 菌株分子身份和相关性的有效工具，将有助于使该技术合法化，并使 FCM 能够提高流行性 MRSA 克隆和其他致病细菌克隆的识别速度。此外，理论上，在我们地区建立强大的分子流行病学/细菌分型单位可以负责识别潜在细菌生物武器的爆发，并用于在相对较短的时间内追踪此类感染的来源。此外，这项试点研究将在 LANL 的 Babetta Marrone 实验室（正在开发尖端细菌分型/识别技术）和 NMSU 教师之间建立牢固的关系，其中一些技术可以在现实世界中进行测试。