STUDIES ON ENZYMES INVOLVED IN THE GLOBAL CYCLING OF HYDROGEN, NITROGEN AND CARB

参与氢、氮和碳水化合物全球循环的酶的研究

• 批准号: 7721997
• 负责人: JOHN W PETERS
• 金额: $ 0.65万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721997
• 关键词: Address; Alkenes; Biochemical; Catalysis; Computer Retrieval of Information on Scientific Projects Database; Enzymes; Funding; Goals; Grant; Hydrogen; Hydrolysis; Individual; Institution; Ketones; Methods; Nitrogen; Pathway interactions; Production; Reaction; Research; Research Personnel; Resolution; Resources; Source; Structure; United States National Institutes of Health; base; enzyme mechanism; enzyme structure; novel; phosphate ester; programs; research study; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The following program proposal narrative describes individual experiments for several projects that involve the use of synchrotron radiation for structure determination using x-ray diffraction methods to address problems concerning the mechanism of enzyme catalyzed reactions. Toward these ends, we are determining the structure of novel enzymes and utilizing information obtained by previous biochemical and biophysical studies as a basis for capturing and determining the structure of enzymes in defined states that mimic intermediates along the pathway of catalysis. The overall goal of these studies is to utilize the structures of defined states of certain enzymes to contribute to the overall understanding of enzyme mechanism and therefore there is significant value in conducting these studies at high resolution. We are currently pursuing these studies for enzymes involved in hydrogen production, nitrogen reduction, phosphate ester hydrolysis, and bioconversion of alkenes and ketones.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 以下项目提案叙述描述了几个项目的单独实验，这些项目涉及使用同步加速器辐射使用 X 射线衍射方法进行结构测定，以解决有关酶催化反应机制的问题。 为此，我们正在确定新型酶的结构，并利用以前的生物化学和生物物理研究获得的信息作为捕获和确定模拟催化途径中间体的特定状态下酶的结构的基础。 这些研究的总体目标是利用某些酶的确定状态的结构来促进对酶机制的整体理解，因此以高分辨率进行这些研究具有重要价值。 我们目前正在对参与制氢、氮还原、磷酸酯水解以及烯烃和酮的生物转化的酶进行这些研究。