STUDIES ON ENZYMES INVOLVED IN THE GLOBAL CYCLING OF HYDROGEN, NITROGEN AND CARB

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 下面的方案提案描述了几个项目的个别实验，这些项目涉及使用同步辐射进行结构测定，使用X射线衍射法来解决与酶催化反应的机理有关的问题。为此，我们正在确定新型酶的结构，并利用先前生化和生物物理研究中获得的信息作为捕获和确定酶在特定状态下的结构的基础，这些酶模仿催化过程中的中间体。这些研究的总体目标是利用某些酶的特定状态的结构来促进对酶机制的全面理解，因此在高分辨率下进行这些研究具有重要的价值。我们目前正在进行这些酶的研究，这些酶涉及产氢、氮还原、磷酸酯水解以及烯烃和酮的生物转化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The following program proposal narrative describes individual experiments for several projects that involve the use of synchrotron radiation for structure determination using x-ray diffraction methods to address problems concerning the mechanism of enzyme catalyzed reactions. Toward these ends, we are determining the structure of novel enzymes and utilizing information obtained by previous biochemical and biophysical studies as a basis for capturing and determining the structure of enzymes in defined states that mimic intermediates along the pathway of catalysis. The overall goal of these studies is to utilize the structures of defined states of certain enzymes to contribute to the overall understanding of enzyme mechanism and therefore there is significant value in conducting these studies at high resolution. We are currently pursuing these studies for enzymes involved in hydrogen production, nitrogen reduction, phosphate ester hydrolysis, and bioconversion of alkenes and ketones.