CHARACTERIZATION OF PHOSPHATIDYLGLYCEROL IN PLANT CHLOROPLASTS BY

植物叶绿体中磷脂酰甘油的表征

• 批准号: 7721501
• 负责人: FONG-FU HSU
• 金额: $ 0.83万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721501
• 关键词: Arabidopsis; Chloroplasts; Computer Retrieval of Information on Scientific Projects Database; Dissociation; Fatty Acids; Funding; Grant; Hydrogen; Institution; Ions; Lipids; Mass Spectrum Analysis; Mouse-ear Cress; Phosphatidyl glycerol; Phosphatidylglycerols; Phospholipids; Plants; Positioning Attribute; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health; ketene; mass spectrometer; progesterone 11-hemisuccinate-(2-iodohistamine)

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Phosphatidylglycerol (PG) is the major phospholipid of plant chloroplasts. PG from Arabidopsis thaliana has an unusual fatty acyl chain, 3,4-trans-hexdecenoyl (D316:1) in the sn-2 position of the major 18:3/D316:1-PG species, as well as in 18:2/D316:1-PG and 16:0/ D316:1-PG. Upon low-energy collisionally activated dissociation (CAD) in a tandem quadrupole or in an ion-trap mass spectrometer, the [M - H] ions of the PG molecules, containing D316:1-PG give product-ion spectra that are readily distinguishable from those arising from PGs without the D316:1 species. The D316:1-fatty acid-containing PGs are characterized by MS2 product-ion mass spectra that contain predominant [M - H - 236], ions arising from loss of the D316:1-fatty acid substituent as a ketene. This is attributable to the fact that the ¿-hydrogen of the D316:1-fatty acid substituent involved in the ketene loss is an allylic hydrogen, which is very labile. This leads to preferential neutral loss of 236 and provides a sensitive tandem quadrupole mass spectrometric means to identify D316:1-containing PG species in lipid mixtures. The low-energy tandem mass spectrometric approach also permits the structures of the Arabidopsis PGs that consist of two isomeric structures to be unveiled.

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，但不一定是研究者所在的机构。 磷脂酰甘油（PG）是植物叶绿体的主要磷脂。来自拟南芥的PG具有不寻常的脂肪酰基链，在主要18：3/D316：1-PG种类以及18：2/D316：1-PG和16：0/ D316：1-PG的sn-2位置处的3，4-反式-己烯酰基（D316：1）。 含有D316：1-PG的PG分子的离子产生的产物离子光谱容易与不含D316：1物质的PG产生的产物离子光谱区分开。 含D316：1-脂肪酸的PG通过MS 2产物离子质谱进行表征，其中主要含有[M-H- 236]，由于D316：1-脂肪酸取代基作为乙烯酮的损失而产生的离子。这是由于参与烯酮损失的D316：1-脂肪酸取代基的？-氢是烯丙基氢，这是非常不稳定的。这导致236的优先中性损失，并提供了一个敏感的串联四极杆质谱手段，以确定脂质混合物中含有D316：1的PG种类。低能量串联质谱的方法也允许由两个异构体结构的拟南芥PG的结构被揭开。