MICROFLUORESCENCE TOMOGRAPHY OF TUMOR TISSUE

肿瘤组织的微荧光断层扫描

• 批准号: 7722771
• 负责人: RAUL BARREA
• 金额: $ 1.9万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722771
• 关键词: Blood capillaries; Computer Retrieval of Information on Scientific Projects Database; Data; Fluorescence; Funding; Grant; Institution; Normal tissue morphology; Numbers; Optics; Research; Research Personnel; Resources; Sampling; Signal Transduction; Source; Spottings; Staging; System; Thick; Time; Tissue Sample; Tumor Tissue; United States National Institutes of Health; beamline; capillary; research study; tomography; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We performed a first attempt at micro fluorescence tomography on tumor and normal tissue samples. The setup used the newly developed optics that combines a single-bounce capillary in combination with the main beamline optics. This arrangement delivers 10 times more flux than the current KB mirrors and provides a focal spot of ~10 microns. The samples are normal and tumor tissue samples 60 microns thick. The samples were mounted on an XY¿ stage to generate the tomography data. In the first part of the experiment we excited the sample with 12 keV energy and we collected fluorescence signals from Ca, Fe, Cu and Zn simultaneously. These data will be use to reconstruct the elemental 3D distribution within the samples. The data collected is still under analysis. We found a number of deficiencies in the system that will require some additional effort. This first attempt indicates the feasibility of this kind of experiment at the BioCAT microprobe although the system is not yet optimized.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们进行了第一次尝试在肿瘤和正常组织样本的显微荧光断层扫描。该装置使用了新开发的光学器件，该光学器件将单反射毛细管与主光束线光学器件相结合。这种布置提供比当前KB镜多10倍的通量，并提供约10微米的焦斑。样品是60微米厚的正常和肿瘤组织样品。将样品安装在XY台上以生成断层扫描数据。在实验的第一部分中，我们用12 keV的能量激发样品，同时收集Ca，Fe，Cu和Zn的荧光信号。这些数据将用于重建样品内的元素3D分布。收集的数据仍在分析中。我们发现该系统存在一些缺陷，需要进一步努力。这第一次尝试表明这种实验的可行性在BioCAT微探针，虽然该系统尚未优化。