COPPER SPECIATION IN PROSTATE & BREAST CANCER

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们使用新设计的Bent Laue分析仪进行了一系列测量，用于CuK边XANES测量。该分析仪由铝弯曲系统和不同厚度的Si(111)晶体组成，以提供更多的熔剂或更高的能量分辨率，具体取决于它们的最终应用。我们已经使用了所选的分析仪，它在能量分辨率方面表现出更好的性能，在CuK？线能量下接近10 eV。我们测量了一组由不同铜氧化态的铜化合物组成的标准样品：铜(I)和铜(II)。使用硅漂移检测器和弯曲劳厄分析仪对这些化合物进行了评估。结果表明，铜(I)化合物在8983 eV处有一个很强的前缘峰，而铜(II)化合物没有该峰。弯曲劳厄分析仪即使在非常稀释的样品条件下也能够识别差异。我们还评估了组织切片中铜的含量：正常组织和肿瘤组织。肿瘤数据表明组织中可能存在铜(II)物种，而正常组织数据表明铜(II)物种的含量很少或没有。这些结果将结合其他补充技术进行评估，以确认肿瘤组织切片中铜(II)的存在。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have performed a series of measurements using the newly designed Bent Laue analyzer for Cu K edge XANES measurements. The analyzers consist of an Al bender system and Si (111) crystal of different thicknesses in order to provide more flux or higher energy resolution depending on their final application. We have used the selected analyzer that shows better performance in term of energy resolution, near 10 eV at the Cu K¿ line energy. We have measured a set of standard samples consisting of Cu compounds of different Cu oxidation state: Cu(I) and Cu(II). These compounds were evaluated using a silicon drift detector and the bent laue analyzer. The results show that Cu(I) compounds have a very strong pre edge peak at 8983 eV where Cu(II) compounds do not have that peak. The bent laue analyzer was able to recognize the difference even under conditions of very diluted samples. We have also evaluated the Cu content of tissue sections: normal and tumor tissue. The tumor data indicates that Cu(II) species are potentially present in the tissue while the normal tissue data indicates very little or no content of Cu(II) species. These results will be evaluated considering other complementary technique to confirm the presence of Cu(II) in tumor tissue sections.