MICRO FLUORESCENCE DEVELOPMENT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们已经实现了一个独立的荧光微探针装置。特定于荧光微探头的组件已组装到30‘x 30’面包板上，该面包板可从主微探针台上移除，以便于与相应的显微衍射设置互换。 我们还测试了一种组合聚焦配置，其中主光束线光学元件用于预聚焦入射光束。这种配置对于一些需要最大传输通量的实验很有用，并且可以接受更大的焦点。根据焦点和KB镜之间的相对位置，预聚焦光束为KB镜产生真实或虚拟的次级源。在这种配置下，我们观察到，仅在水平方向预聚焦光束就可以使传输的光束通量增加3倍。 BioCAT-FMAP扫描软件最近得到了改进，纳入了新的例程，以在连续扫描期间以最小的每像素开销时间优化荧光数据的获取。每个像素的计数时间现在可以低至50毫秒。 此外，软件中还集成了绘图功能。用户现在有几个选项可用于实时绘制测量数据。可预选单图和多图。这些例程已得到改进，以便在扫描期间提供更大的灵活性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have implemented an independent fluorescence microprobe setup. The components specific to the fluorescence microprobe has been assembled onto a 30' x 30' breadboard that can be removed from the main microprobe table for easy interchangeability with the corresponding setup for micro-diffraction. We have also tested a combined focusing configuration where the main beamline optics is used to pre-focus the incident beam. This configuration is useful for some experiments that required maximum delivered flux and can accept larger focal spots. The pre-focused beam generates a real or virtual secondary source for the KB mirrors, depending on the relative location between the focal point and the KB mirrors. Under this configuration we have observed that the delivered beam flux can be increased 3 fold by pre-focusing the beam in the horizontal direction only. The BioCAT-FMAP scanning software has been recently improved by incorporating new routines to optimize the acquisition of the fluorescence data with minimum overhead time per pixel during continuous scans. The counting time per pixel can now be as low as 50 milliseconds. Additionally, plotting functionality has been incorporated to the software. The users now have several options for on-the-fly plotting of the measured data. Single plots and multi plots can be pre-selected. These routines have been improved to offer more flexibility during the scanning time.