MICRO FLUORESCENCE DEVELOPMENT

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 我们已经实现了一个独立的荧光微探针设置。荧光微探针专用组件已组装到30' x 30'试验板上，该试验板可从主微探针台上移除，以便与相应的微衍射设置轻松互换。 我们还测试了组合聚焦配置，其中主光束线光学器件用于预聚焦入射光束。这种配置对于一些需要最大输送通量的实验是有用的，并且可以接受更大的焦斑。根据焦点和KB镜之间的相对位置，预聚焦光束为KB镜生成真实的或虚拟的次级源。在这种配置下，我们已经观察到，通过仅在水平方向上预聚焦射束，可以将输送的射束通量增加3倍。 BioCAT-FMAP扫描软件最近经过改进，加入了新的程序，以优化荧光数据的采集，在连续扫描期间每个像素的开销时间最小。每个像素的计数时间现在可以低至50毫秒。 此外，绘图功能已纳入软件。用户现在有几个选项可以实时绘制测量数据。可以预先选择单个地块和多个地块。这些例程已得到改进，以便在扫描期间提供更大的灵活性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have implemented an independent fluorescence microprobe setup. The components specific to the fluorescence microprobe has been assembled onto a 30' x 30' breadboard that can be removed from the main microprobe table for easy interchangeability with the corresponding setup for micro-diffraction. We have also tested a combined focusing configuration where the main beamline optics is used to pre-focus the incident beam. This configuration is useful for some experiments that required maximum delivered flux and can accept larger focal spots. The pre-focused beam generates a real or virtual secondary source for the KB mirrors, depending on the relative location between the focal point and the KB mirrors. Under this configuration we have observed that the delivered beam flux can be increased 3 fold by pre-focusing the beam in the horizontal direction only. The BioCAT-FMAP scanning software has been recently improved by incorporating new routines to optimize the acquisition of the fluorescence data with minimum overhead time per pixel during continuous scans. The counting time per pixel can now be as low as 50 milliseconds. Additionally, plotting functionality has been incorporated to the software. The users now have several options for on-the-fly plotting of the measured data. Single plots and multi plots can be pre-selected. These routines have been improved to offer more flexibility during the scanning time.