MICRODIFFRACTION DEVELOPMENT

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 我们继续对去年安装和调试的200 mm长KB反射镜系统（芝加哥大学设计）的性能感到满意。 从低分辨率到高分辨率的变化与这些镜子可以执行上，在最少的工作人员干预和时间使用的情况下飞行。这一特点大大增加了仪器的多功能性。 最近的其他变化包括新的可调节飞行管（80，150和200毫米长），比以前使用的更坚固，更容易调节。结合更大的检测器（重建的MAR 165）的可用性，这允许仪器能够进行中等和小角度衍射/散射（范围134  3.5¿在最常见的配置中，在最大相机长度下约为300 dmin）我们还重新安排了定位台，以便用户更容易进入样品区。最后，微衍射设置的特定组件现在安装在专用试验板上，因此从微探针切换到微衍射只需几个小时。 对屏蔽寄生散射进行了进一步的改进，现在可以非常迅速地显示弱衍射特性。 为了充分利用这一进展，我们最近集中精力改进冷冻保存技术，迄今取得了一些明显的成功。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We continue to be pleased with the performance of the 200 mm long KB mirror system (University of Chicago design) that was installed and commissioned last year. Changing from low resolution to high resolution with these mirrors can be performed on-thefly with minimum staff intervention and time usage. This feature greatly increases the versatility of the instrument. Other recent changes include new adjustable flight tubes (80, 150 and 200mm lengths) that are much more robust and easier to adjust than the ones used previously. Combined with the availability of a larger detector (the rebuilt MAR 165) this allows the instrument to be capable of both medium and small angle diffraction/scattering (range 134  3.5 ¿ in the most common configuration, ~ 300 ¿ dmin at maximum camera length) We have also rearranged the positioning stages to allow easier access to the sample area by the users. Finally, the components specific to the micro-diffraction setup are now mounted on a dedicated breadboard so that switching from microprobe to microdiffraction only takes a few hours. Further improvements have been made towards shielding parasitic scattering which now enables weak diffraction characteristics to be visualized very rapidly. In order to take full advantage of this progress, we have more recently concentrated on improving cryo-preservation techniques with some appreciable success to date.