Biomarkers of Exposure to Hazardous Substances

计划项目摘要。接触有害物质的生物标志物 尽管近年来我们分析废物场中危险材料的能力有了显著提高， 我们通过各种媒介追踪危险材料从超级基金地点转移的能力非常有限 或者优先考虑和减轻所涉及的危险。我们预测这些材料对人类的暴露或影响的能力 它们的生存环境也更加有限。该计划包括8个综合项目，3个研究支持核心， 一个培训核心、一个研究翻译核心和一个行政核心，以解决这些问题。康贝特人将以 “在地下水、地表水和空气中从有毒废物场移动时， 使用传统和创新的方法。我们将使用 心理学方法与此同时，我们将开发敏感的系统，用于评估 这些材料的人口。将使用免疫化学、基于细胞的系统和其他系统来检测生物标志物。 这些生物标志物的开发将基于对毒理学过程的基本理解 涉案该项目将强调多个器官系统，重点是肺部和生殖系统 方面的影响.我们还将探索有毒废物热处理和生物修复的新技术， 与这些技术相关的潜在风险。快速免疫化学和细胞分析将补充 经典的技术，用于评估网站，验证模型的运输从这些网站，以及确定 流明敏感性、暴露和效应。现代质谱技术将被评估用于监测母体 lazPreferchemicals以及暴露和效应的生物标志物。我们正在扩大转录组学的应用， 代谢组学和综合生物信息学技术，以发现新的作用机制， lazPreferred材料和生物标记物的作用。在这个项目中开发的生物标志物将作为生物 在本项目和姊妹项目的流行病学和生态学研究中使用剂量计。发展中的技术 该项目将在实地进行测试，并通过研究翻译核心转交给最终用户。

Program Project Abstract. Biomarkers of Exposure to Hazardous Substances Although our ability to analyze hazardous material in waste sites has improved dramatically in recent years, we are very limited in our ability to trace the movement of hazardous materials from Superfund sites through various media or to prioritize and mitigate the hazards involved. Our ability to predict exposure or effect of these materials on humans and their environment is still more limited. This Program consists of 8 integrated projects, 3 research support cores, a training core, a research translation core and an administrative core to address these problems. We will determine the "ate and transport of hazardous materials in ground water, surface water, and air as they move from toxic waste sites using classical and innovative methodologies. We will examine the effect of some of these materials using an pidemiological approach. Concurrently we will develop sensitive systems for evaluating the exposure and effect of populations to these materials. Immunochemical, cell based and other systems will be used to detect biomarkers. Development of these biomarkers will be based on a fundamental understanding of the toxicological processes involved. The project will emphasize multiple organsystems with an in vivo emphasis on pulmonary and reproductive effects. We also will explore new technologies for thermal and bioremediation of toxic waste and address possible lealth risks associated with these technologies. Rapid immunochemical and cell based analysis will supplement classical technologies for the evaluation of sites, validating models of transport from these sites, as well as determining luman susceptibility, exposure and effect. Modern mass spectral technology will be evaluated for monitoring parent lazardous chemicals as well as biomarkers of exposure and effect. We are expanding the use of transcriptomics, aroteomics, metabolomics and integrated bio informatics technologies to discover new mechanisms of action of lazardous materials and biomarkers for their action. The biomarkers developed in this project will serve as biological dosimeters in epidemiological and ecological studies in this and sister projects. The technologies developed in the project will be tested at field sites and transferred to end users through a research translation core.