CELL MIGRATION CONSORTIUM PROJECT

细胞迁移联盟项目

• 批准号: 7724030
• 负责人: ENRICO GRATTON
• 金额: $ 10.48万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724030
• 关键词: Adhesions; Color; Computer Retrieval of Information on Scientific Projects Database; Funding; Goals; Grant; Image; Image Analysis; Institution; Measures; Methods; Microscopy; Molecular; Movement; Protein Binding; Rate; Research; Research Personnel; Resources; Signal Transduction; Source; Time; United States National Institutes of Health; analog; cell motility; protein aggregation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The primary goals for correlation microscopy are to develop a toolbox for analyzing image sequences for: a) extracting protein binding dynamics using raster image correlation (RICS) and TIRF time correlation microscopy, b) identifying molecular associations by two color cross-correlation methods, c) determining protein aggregation states by intensity brightness analysis of analog signals, and d) developing spatial-temporal correlation analyses for measuring adhesion assembly and disassembly rates and rates and directions of movement.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 相关显微镜的主要目标是开发一个用于分析图像序列的工具箱，用于：a) 使用光栅图像相关 (RICS) 和 TIRF 时间相关显微镜提取蛋白质结合动力学，b) 通过两种颜色互相关方法识别分子关联，c) 通过模拟信号的强度亮度分析确定蛋白质聚集状态，以及 d) 开发时空相关分析 用于测量粘附组装和拆卸速率以及运动速率和方向。