MOMENT ANALYSIS TO DETERMINE PROTEIN CLUSTERING & NUMBER COUNT IN LIVE CELLS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们正在开发一种时刻分析技术，该技术可以用来确定溶液中和活细胞中的蛋白质数量和聚集。这一理论已被用于光子计数和模拟系统。光子计数系统能够以高精度恢复绝对数和亮度信息，而模拟系统可以潜在地恢复精确值，前提是探测器能够正确地表征并在测量过程中保持稳定。最后，也可以区分固定分数和流动分数。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are developing a moment analysis technique which can be used to determine protein number and clustering in solution as well as in live cells. The theory has been developed for both photon counting and analog systems. Photon counting systems are able to recover absolute number and brightness information with high accuracy, whereas analog systems can potentially recover accurate values provided the detector can be properly characterized and remains stable during the course of the measurement. Lastly, immobile and mobile fractions can be distinguished as well.