Cell Migration Consortium

细胞迁移联盟

• 批准号: 7482412
• 负责人: ALAN F. Rick HORWITZ
• 金额: $ 666.93万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-15 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7482412
• 关键词: Cell; Migration; Consortium

DESCRIPTION (provided by applicant): Migration is a pivotal process in many phenomena including embryonic development, cancer, inflammation, and wound repair. Understanding the mechanisms of cell migration present a formidable challenge, since it is comprised of several complex cellular processes coordinated in space and time. The Cell Migration Consortium is a highly interactive, multidisciplinary effort focused on bringing novel technical and intellectual solutions to the barriers to progress in migration research. It is organized into interdisciplinary Initiatives, virtual collaborative laboratories, focused on developing enabling technologies, reagents and models to address these barriers to progress. In the Consortium's next phase the emphasis will shift from technology development to its use in addressing major questions in the field. The Consortium goals are: to determine, identify, catalogue, and characterize the migration proteome, 2) formulate mathematical models to describe the steps in migration and integrate them into a comprehensive model of migration, 3) establish reagents and methods to measure, activate, and inactivate local signals that regulate migration, 4) determine the structures of the large multi-molecular machines that drive migration, and 5) promote migration research in the community and lower barriers for entry into the field. To achieve these goals enabling technologies developed by the individual Initiatives will be shared across the Consortium via inter-Initiative collaborations both inside and outside the migration community. The Consortium is organized into 7 Initiatives. Proteomics (mass spectrometry based analyses of phosphorylation and binding partners), Discovery (expression assays to identify migration molecules in several organisms), Transgenic and Knockout Mouse (production of conditional knockout and RNAi knockdown mice), Structure (determination of high resolution structures of migration complexes), Biosensors (development and application of new biosensors to measure spatial signaling), Imaging and Photomanipulation (development and application of photoactivation, photoinactivation, traction force assays, and correlation microscopy), Modeling (develop mathematical models of cell migration), and a Data Coordination and Dissemination Core (organize information for the Consortium and the community. The Consortium resources will be disseminated to the Community via the Cell Migration Gateway, a collaboration with the Nature Publishing Group.

描述（由申请人提供）：迁移是许多现象的关键过程，包括胚胎发育、癌症、炎症和伤口修复。了解细胞迁移的机制是一个艰巨的挑战，因为它由在空间和时间上协调的几个复杂的细胞过程组成。细胞迁移联盟是一个高度互动、多学科的组织，致力于为迁移研究进展的障碍带来新颖的技术和知识解决方案。它由跨学科倡议、虚拟协作实验室组成，专注于开发支持技术、试剂和模型，以解决这些进展障碍。在联盟的下一阶段，重点将从技术开发转向利用技术解决该领域的重大问题。该联盟的目标是：确定、识别、编目和表征迁移蛋白质组，2) 制定数学模型来描述迁移步骤并将其整合到迁移的综合模型中，3) 建立试剂和方法来测量、激活和灭活调节迁移的局部信号，4) 确定驱动迁移的大型多分子机器的结构，以及 5) 促进社区和下游的迁移研究。 进入该领域的壁垒。为了实现这些目标，各个倡议开发的技术将通过移民社区内外的倡议间合作在联盟内共享。该联盟分为 7 个项目。蛋白质组学（基于质谱分析的磷酸化和结合伴侣）、发现（用于识别多种生物体中迁移分子的表达测定）、转基因和敲除小鼠（条件敲除和 RNAi 敲除小鼠的生产）、结构（迁移复合物的高分辨率结构的确定）、生物传感器（用于测量空间信号传导的新型生物传感器的开发和应用）、成像和 光操纵（光活化、光失活、牵引力测定和相关显微镜的开发和应用）、建模（开发细胞迁移的数学模型）以及数据协调和传播核心（为联盟和社区组织信息。联盟资源将通过与自然出版集团合作的细胞迁移网关传播到社区。