Cell Migration Consortium

细胞迁移联盟

• 批准号: 7664918
• 负责人: ALAN F. Rick HORWITZ
• 金额: $ 666.68万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-15 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7664918
• 关键词: Cell; Migration; Consortium

DESCRIPTION (provided by applicant): Migration is a pivotal process in many phenomena including embryonic development, cancer, inflammation, and wound repair. Understanding the mechanisms of cell migration present a formidable challenge, since it is comprised of several complex cellular processes coordinated in space and time. The Cell Migration Consortium is a highly interactive, multidisciplinary effort focused on bringing novel technical and intellectual solutions to the barriers to progress in migration research. It is organized into interdisciplinary Initiatives, virtual collaborative laboratories, focused on developing enabling technologies, reagents and models to address these barriers to progress. In the Consortium's next phase the emphasis will shift from technology development to its use in addressing major questions in the field. The Consortium goals are: to determine, identify, catalogue, and characterize the migration proteome, 2) formulate mathematical models to describe the steps in migration and integrate them into a comprehensive model of migration, 3) establish reagents and methods to measure, activate, and inactivate local signals that regulate migration, 4) determine the structures of the large multi-molecular machines that drive migration, and 5) promote migration research in the community and lower barriers for entry into the field. To achieve these goals enabling technologies developed by the individual Initiatives will be shared across the Consortium via inter-Initiative collaborations both inside and outside the migration community. The Consortium is organized into 7 Initiatives. Proteomics (mass spectrometry based analyses of phosphorylation and binding partners), Discovery (expression assays to identify migration molecules in several organisms), Transgenic and Knockout Mouse (production of conditional knockout and RNAi knockdown mice), Structure (determination of high resolution structures of migration complexes), Biosensors (development and application of new biosensors to measure spatial signaling), Imaging and Photomanipulation (development and application of photoactivation, photoinactivation, traction force assays, and correlation microscopy), Modeling (develop mathematical models of cell migration), and a Data Coordination and Dissemination Core (organize information for the Consortium and the community. The Consortium resources will be disseminated to the Community via the Cell Migration Gateway, a collaboration with the Nature Publishing Group.

描述（由申请人提供）：迁移是许多现象的关键过程，包括胚胎发育、癌症、炎症和伤口修复。了解细胞迁移的机制是一个巨大的挑战，因为它是由几个复杂的细胞过程在空间和时间上协调。细胞迁移联盟是一个高度互动的，多学科的努力，重点是带来新的技术和智力解决方案的障碍，在迁移研究的进展。它被组织成跨学科的倡议，虚拟合作实验室，重点是开发使能技术，试剂和模型，以解决这些进展的障碍。在联合会的下一阶段，重点将从技术开发转向利用技术解决该领域的主要问题。联合会的目标是：确定、鉴定、分类和表征迁移蛋白质组，2）制定数学模型来描述迁移步骤并将其整合到迁移的综合模型中，3）建立试剂和方法来测量、激活和检测调节迁移的局部信号，4）确定驱动迁移的大型多分子机器的结构，五是推动社区移民研究，降低进入该领域的门槛。为了实现这些目标，各个倡议开发的使能技术将通过移民社区内外的倡议间合作在联盟内共享。该联盟分为7个倡议。Proteomics（基于质谱的磷酸化和结合伴侣分析），Discovery（表达测定法，用于鉴定几种生物体中的迁移分子），转基因和敲除小鼠（条件性敲除和RNAi敲除小鼠的产生），结构（测定迁移复合物的高分辨率结构），生物传感器（开发和应用新的生物传感器来测量空间信号），成像和光操纵（光活化、光失活、牵引力测定和相关显微镜的开发和应用），建模（开发细胞迁移的数学模型），以及数据协调和传播核心（为联盟和社区组织信息）。该联盟的资源将通过与自然出版集团合作的细胞迁移网关传播给社区。