Cell Migration Consortium

细胞迁移联盟

• 批准号: 7664918
• 负责人: ALAN F. Rick HORWITZ
• 金额: $ 666.68万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-15 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7664918
• 关键词: Cell; Migration; Consortium

DESCRIPTION (provided by applicant): Migration is a pivotal process in many phenomena including embryonic development, cancer, inflammation, and wound repair. Understanding the mechanisms of cell migration present a formidable challenge, since it is comprised of several complex cellular processes coordinated in space and time. The Cell Migration Consortium is a highly interactive, multidisciplinary effort focused on bringing novel technical and intellectual solutions to the barriers to progress in migration research. It is organized into interdisciplinary Initiatives, virtual collaborative laboratories, focused on developing enabling technologies, reagents and models to address these barriers to progress. In the Consortium's next phase the emphasis will shift from technology development to its use in addressing major questions in the field. The Consortium goals are: to determine, identify, catalogue, and characterize the migration proteome, 2) formulate mathematical models to describe the steps in migration and integrate them into a comprehensive model of migration, 3) establish reagents and methods to measure, activate, and inactivate local signals that regulate migration, 4) determine the structures of the large multi-molecular machines that drive migration, and 5) promote migration research in the community and lower barriers for entry into the field. To achieve these goals enabling technologies developed by the individual Initiatives will be shared across the Consortium via inter-Initiative collaborations both inside and outside the migration community. The Consortium is organized into 7 Initiatives. Proteomics (mass spectrometry based analyses of phosphorylation and binding partners), Discovery (expression assays to identify migration molecules in several organisms), Transgenic and Knockout Mouse (production of conditional knockout and RNAi knockdown mice), Structure (determination of high resolution structures of migration complexes), Biosensors (development and application of new biosensors to measure spatial signaling), Imaging and Photomanipulation (development and application of photoactivation, photoinactivation, traction force assays, and correlation microscopy), Modeling (develop mathematical models of cell migration), and a Data Coordination and Dissemination Core (organize information for the Consortium and the community. The Consortium resources will be disseminated to the Community via the Cell Migration Gateway, a collaboration with the Nature Publishing Group.

描述(申请人提供)：迁移是许多现象的关键过程，包括胚胎发育、癌症、炎症和伤口修复。理解细胞迁移的机制是一项艰巨的挑战，因为它由几个在空间和时间上协调的复杂细胞过程组成。细胞迁移联盟是一个高度互动的多学科努力，专注于为移民研究进展的障碍带来新的技术和智力解决方案。它被组织成跨学科倡议、虚拟协作实验室，重点是开发使能技术、试剂和模型，以消除这些阻碍进展的障碍。在该联盟的下一阶段，重点将从技术开发转向利用技术来解决该领域的主要问题。该联盟的目标是：确定、识别、编目和表征迁移蛋白质组；2)建立描述迁移步骤的数学模型，并将其集成到一个全面的迁移模型中；3)建立试剂和方法来测量、激活和失活调节迁移的局部信号；4)确定驱动迁移的大型多分子机器的结构；以及5)促进社区中的迁移研究，降低进入该领域的门槛。为了实现这些目标，将通过在移民界内外的倡议间协作，在联盟内共享由各个倡议开发的使能技术。该联盟由7项倡议组成。蛋白质组学(基于质谱学的磷酸化和结合伙伴分析)，Discovery(确定几个生物中迁移分子的表达分析)，转基因和RNAi敲除小鼠(产生条件基因敲除和RNAi敲除小鼠)，结构(确定迁移复合体的高分辨率结构)，生物传感器(开发和应用新型生物传感器来测量空间信号)，成像和光操纵(光活化、光灭活、牵引力分析和相关显微镜的发展和应用)，建模(建立细胞迁移的数学模型)，以及数据协调和传播核心(为财团和社区组织信息。该联盟的资源将通过与自然出版集团合作的细胞迁移网关传播给社区。