THE ULTASTRUCTURAL LOCALIZATION OF MINERAL WITHIN THE COLLAGEN FIBRIL

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们之前已经证明，肌腱和脱钙骨在37℃的大鼠血清中孵育后迅速重新钙化。本研究的目的是检测在血清中孵育过程中沉积在这些1型胶原基质中的初始矿物质的超微结构位置。我们的工作假设是，最初的矿物质将沉积在胶原纤维的孔洞区域，就像在体内矿化的初始阶段所看到的那样。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have previously shown that tendon and demineralized bone re-calcify rapidly when incubated at 37C in rat serum (1). The objective of the present study is to examine the ultrastructural location of the initial mineral deposited into these type 1 collagen matrices during incubation in serum. Our working hypothesis is that the initial mineral will be deposited within the hole region of the collagen fibril, as has been seen in the initial stages of mineralization in vivo.