Simultaneous Stimulation and Recording in Scalable Microelectrode Arrays

可扩展微电极阵列中的同步刺激和记录

• 批准号: 8058252
• 负责人: James Ross
• 金额: $ 53.22万
• 依托单位: AXION BIOSYSTEMS, LLC
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-15 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8058252
• 关键词: Simultaneous; Stimulation; Recording; Scalable; Microelectrode

DESCRIPTION (provided by applicant): The proposed study utilizes novel electronics and microfabrication techniques to create scalable, in-vitro Microelectrode Array (MEA) technologies that conform to industry standards for multiwell plates. This research will not only enable rapid advancements in the study of network-level electrophysiology, but it will also create new opportunities for pharmaceutical research and toxicity screening. This Phase II proposal involves two significant developments for neurological research. Specifically, Aim 1 builds on the scalable simultaneous stimulation and recording Integrated Circuit (IC) developed in Phase I to produce a full electronics platform for capturing, processing and storing electrophysiological information. Among its many advantages, this electronics platform will recover signals traditionally obscured by stimulation artifacts. This captured data, combined with the ability to simultaneously manage 768 microelectrodes and automate experimental protocols, will provide new measures of single-cell and network-level neural activity. Aim 2 will produce scalable, inexpensive, and flexible processes for fabricating multiwell MEAs that, in conjunction with the electronics developed in Aim 1, will yield a high-throughput, network-electrophysiology toolset. PUBLIC HEALTH RELEVANCE: This research uses novel electronic and fabrication technologies to create faster, lower-cost methods for neural research. Ultimately, this development will facilitate medical and scientific discoveries that will benefit the treatment of neural disorders such as Parkinson's disease and epilepsy.

描述（由申请人提供）：拟议的研究利用新颖的电子和微加工技术来创建符合多孔板行业标准的可扩展的体外微电极阵列（MEA）技术。这项研究不仅将推动网络级电生理学研究的快速进步，而且还将为药物研究和毒性筛选创造新的机会。该第二阶段提案涉及神经学研究的两项重大进展。具体来说，Aim 1 建立在第一阶段开发的可扩展同步刺激和记录集成电路 (IC) 的基础上，以生成用于捕获、处理和存储电生理信息的完整电子平台。该电子平台具有众多优点，其中之一是可以恢复传统上被刺激伪影掩盖的信号。捕获的数据与同时管理 768 个微电极和自动化实验方案的能力相结合，将提供单细胞和网络级神经活动的新测量方法。目标 2 将产生用于制造多孔 MEA 的可扩展、廉价且灵活的工艺，与目标 1 中开发的电子器件相结合，将产生高通量的网络电生理学工具集。 公共健康相关性：这项研究使用新颖的电子和制造技术来创建更快、成本更低的神经研究方法。最终，这一发展将促进医学和科学发现，有利于帕金森病和癫痫等神经疾病的治疗。