Simultaneous Stimulation and Recording in Scalable Microelectrode Arrays

描述（由申请人提供）：拟议的研究利用新颖的电子和微加工技术来创建可扩展的体外微电极阵列（MEA）技术，该技术符合多孔板的行业标准。这项研究不仅将使网络级电生理学的研究取得快速进展，而且还将为药物研究和毒性筛选创造新的机会。该二期计划涉及神经学研究的两项重大进展。具体来说，Aim 1建立在第一阶段开发的可扩展同步刺激和记录集成电路（IC）的基础上，以产生一个完整的电子平台，用于捕获、处理和存储电生理信息。在众多优势中，该电子平台将恢复传统上被刺激伪影掩盖的信号。这些捕获的数据，结合同时管理768个微电极和自动化实验协议的能力，将提供单细胞和网络级神经活动的新测量方法。Aim 2将为制造多井mea提供可扩展、廉价和灵活的工艺，与Aim 1中开发的电子技术相结合，将产生高通量的网络电生理工具集。

DESCRIPTION (provided by applicant): The proposed study utilizes novel electronics and microfabrication techniques to create scalable, in-vitro Microelectrode Array (MEA) technologies that conform to industry standards for multiwell plates. This research will not only enable rapid advancements in the study of network-level electrophysiology, but it will also create new opportunities for pharmaceutical research and toxicity screening. This Phase II proposal involves two significant developments for neurological research. Specifically, Aim 1 builds on the scalable simultaneous stimulation and recording Integrated Circuit (IC) developed in Phase I to produce a full electronics platform for capturing, processing and storing electrophysiological information. Among its many advantages, this electronics platform will recover signals traditionally obscured by stimulation artifacts. This captured data, combined with the ability to simultaneously manage 768 microelectrodes and automate experimental protocols, will provide new measures of single-cell and network-level neural activity. Aim 2 will produce scalable, inexpensive, and flexible processes for fabricating multiwell MEAs that, in conjunction with the electronics developed in Aim 1, will yield a high-throughput, network-electrophysiology toolset. PUBLIC HEALTH RELEVANCE: This research uses novel electronic and fabrication technologies to create faster, lower-cost methods for neural research. Ultimately, this development will facilitate medical and scientific discoveries that will benefit the treatment of neural disorders such as Parkinson's disease and epilepsy.