Biophysical Instrumentation & Protein Purification Core

生物物理仪器和蛋白质纯化核心(BIPPC)将协助设计和实施 对所提议的项目至关重要的各种技术。核心目前由七名科学家组成 位于一带一路内，在蛋白质化学和多肽合成领域拥有专业知识。分析了几个问题 蛋白质的结构和蛋白质之间的相互作用将使用各种光谱进行研究。 方法：分析超速离心法、量热法、表面等离子体共振法。核心将提供 蛋白质工程能力，将允许表达所需的蛋白质和蛋白质结构 项目2和项目3中提出的研究。本申请中提出的一些研究；例如，FRET 项目2的研究将由核心内的首席调查组直接进行。在这样的情况下 在情况下，核心成员将在需要时提供支持，如仪器 维护和校准，帮助标记、纯化和蛋白质水解性消化蛋白质，以及培训 新博士后和技术人员正确使用我们的仪器。

The Biophysical Instrumentation & Protein Purification Core (BIPPC) will assist in the design and execution of a variety of techniques essential for the proposed projects. The core consists of seven scientists currently located within BBRI with expertise in the areas of protein chemistry and peptide synthesis. The analysis of protein structure and protein-protein interactions will be approached using a variety of spectroscopic methods, analytical ultracentrifugation, calorimetry, and surface plasmon resonance. The core will provide protein engineering capabilities that will allow the expression of proteins and protein constructs required for studies proposed in projects 2 and 3. Some studies proposed in this application; for example the FRET studies in Project 2 will be carried out directly by the principal investigator's group within the core. In such cases, members of the core will be available to provide support when required such as instrument maintenance and calibration, help in labeling, purification and proteolytic digestion of proteins, and training new post-docs and technicians in the correct use of our instrumentation.