Biophysical Instrumentation & Protein Purification Core

生物物理仪器和蛋白质纯化核心（BIPPC）将协助设计和执行 一系列的技术对项目的实施至关重要。核心由七名科学家组成， 位于BBRI，在蛋白质化学和肽合成领域拥有专业知识。分析 蛋白质结构和蛋白质-蛋白质相互作用将采用各种光谱方法进行处理。 方法，分析超离心，量热法和表面等离子体共振。核心将提供 蛋白质工程能力，这将允许表达所需的蛋白质和蛋白质构建体， 项目2和项目3中提出的研究。本申请中提出的一些研究;例如FRET 项目2中的研究将由核心小组内的主要研究者小组直接进行。以这样 在某些情况下，核心成员将在需要时提供支持，例如 维护和校准，帮助标记，纯化和蛋白质的蛋白水解消化，以及培训 新的博士后和技术人员正确使用我们的仪器。

The Biophysical Instrumentation & Protein Purification Core (BIPPC) will assist in the design and execution of a variety of techniques essential for the proposed projects. The core consists of seven scientists currently located within BBRI with expertise in the areas of protein chemistry and peptide synthesis. The analysis of protein structure and protein-protein interactions will be approached using a variety of spectroscopic methods, analytical ultracentrifugation, calorimetry, and surface plasmon resonance. The core will provide protein engineering capabilities that will allow the expression of proteins and protein constructs required for studies proposed in projects 2 and 3. Some studies proposed in this application; for example the FRET studies in Project 2 will be carried out directly by the principal investigator's group within the core. In such cases, members of the core will be available to provide support when required such as instrument maintenance and calibration, help in labeling, purification and proteolytic digestion of proteins, and training new post-docs and technicians in the correct use of our instrumentation.