权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Biophysical Instrumentation & Protein Purification Core

生物物理仪器

基本信息

批准号：
8079497
负责人：
PAUL Clifton LEAVIS
金额：
$ 12.92万
依托单位：
BOSTON UNIVERSITY (CHARLES RIVER CAMPUS)
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8079497
关键词：
Acids Alanine Amines Amino Acids Area Binding Biotin Biotinylation Calibration Calorimetry Chemistry Cleaved cell Complex Coupled Coupling Custom Cysteine Digestion Dimethyl Sulfoxide Equilibrium Equipment Esters Excision Fluorescence Resonance Energy Transfer High Pressure Liquid Chromatography Homologous Gene Human Resources Isothiocyanates Label Laws Left Lysine MALDI-TOF Mass Spectrometry Maintenance Measurement Measures Methods Modeling Monitor N-terminal Peptide Synthesis Peptides Phase Phenols Phosphopeptides Plant Resins Postdoctoral Fellow Principal Investigator Production Property Protein Chemistry Protein Engineering Proteins Randomized Reagent Sampling Scientist Sedimentation process Services Side Site Solutions Specificity Structure Sulfhydryl Compounds Surface Surface Plasmon Resonance System Techniques Testing Thermodynamics Time Training Trifluoroacetic Acid Work amino group analytical ultracentrifugation benzotriazole carboxyl group design experience instrument instrumentation macromolecule mass spectrometer member protein aminoacid sequence protein expression protein protein interaction protein purification protein structure research study solid state synthetic peptide

项目摘要

The Biophysical Instrumentation & Protein Purification Core (BIPPC) will assist in the design and execution of a variety of techniques essential for the proposed projects. The core consists of seven scientists currently located within BBRI with expertise in the areas of protein chemistry and peptide synthesis. The analysis of protein structure and protein-protein interactions will be approached using a variety of spectroscopic methods, analytical ultracentrifugation, calorimetry, and surface plasmon resonance. The core will provide protein engineering capabilities that will allow the expression of proteins and protein constructs required for studies proposed in projects 2 and 3. Some studies proposed in this application; for example the FRET studies in Project 2 will be carried out directly by the principal investigator's group within the core. In such cases, members of the core will be available to provide support when required such as instrument maintenance and calibration, help in labeling, purification and proteolytic digestion of proteins, and training new post-docs and technicians in the correct use of our instrumentation.

生物物理仪器和蛋白质纯化核心（BIPPC）将协助设计和执行一系列的技术对项目的实施至关重要。核心由七名科学家组成，位于BBRI，在蛋白质化学和肽合成领域拥有专业知识。分析蛋白质结构和蛋白质-蛋白质相互作用将采用各种光谱方法进行处理。方法，分析超离心，量热法和表面等离子体共振。核心将提供蛋白质工程能力，这将允许表达所需的蛋白质和蛋白质构建体，项目2和项目3中提出的研究。本申请中提出的一些研究;例如FRET 项目2中的研究将由核心小组内的主要研究者小组直接进行。以这样在某些情况下，核心成员将在需要时提供支持，例如维护和校准，帮助标记，纯化和蛋白质的蛋白水解消化，以及培训新的博士后和技术人员正确使用我们的仪器。