REGULATION OF CARDIAC AND SKELETAL MUSCLE CONTRACTION

调节心脏和骨骼肌收缩

• 批准号: 3336148
• 负责人: PAUL Clifton LEAVIS
• 金额: $ 19.77万
• 依托单位: BOSTON BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-07-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3336148
• 关键词: actins; analytical method; binding proteins; biological information processing; calcium binding protein; calmodulin; chemical group; chemical kinetics; conformation; crosslink; fluorescence spectrometry; heart contraction; laboratory rabbit; metal complex; molecular rearrangement; muscle contraction; myocardium; myofibrils; myosins; nuclear magnetic resonance spectroscopy; nucleotide analog; nucleotides; protein structure; proteolysis; striated muscles; tropomyosin; troponin

The molecular mechanisms by which troponin and tropomyosin regulate the interaction of actin and myosin in skeletal muscle will be investigated, involving a detailed study of the changes in structure and interactions among the troponin subunits and the other thin filament proteins, actin and tropomyosin. This will be approached by studying a) details of the strength and sites of intersubunit binding between TnC, TnI and TnT; b) the transfer of metal-induced structural information to proteins forming complexes with TnC; c) the effects of actin-myosin interactions on the cooperativity of Ca2+ binding to troponin. The major experimental tools for these studies will be optical and magnetic spectroscopy and hydrogen/tritium exchange studies. It is expected that the study presented in this proposal may contribute to an understanding of the regulatory processes in muscles. New knowledge developed here on metal-protein and protein-protein interactions should be of significance in the general fied of protein chemistry since similar processes are widely distributed among biological systems in a variety of physiological processes. Finally, knowledge about normal muscle function is essential for the eventual understanding of the molecular mechanisms underlying disease processes in muscle.

肌钙蛋白和原肌球蛋白调节血管紧张素转换酶的分子机制 肌动蛋白和肌球蛋白在骨骼肌中的相互作用将被研究， 包括对结构变化和相互作用的详细研究 在肌钙蛋白亚基和其他细丝蛋白中，肌动蛋白和 原肌球蛋白。这将通过研究a)详细的 TnC、TnI和TnT之间亚基间结合的强度和位置；b) 金属诱导的结构信息向蛋白质形成的转移 肌动蛋白-肌球蛋白相互作用对TNC的影响 钙离子与肌钙蛋白结合的协同性。主要实验工具 因为这些研究将是光学和磁光谱学以及 氢/氚交换研究。预计这项研究将提交 这一建议可能有助于理解监管 肌肉中的过程。在这里开发了关于金属-蛋白质和 蛋白质之间的相互作用在一般情况下应该是重要的 因为相似的过程广泛分布在 生物系统中的各种生理过程。最后， 关于正常肌肉功能的知识对于最终的 对疾病过程中潜在分子机制的理解 肌肉。

项目成果

Proton magnetic resonance studies on peptide fragments of troponin-C containing single calcium-binding sites.

对含有单个钙结合位点的肌钙蛋白-C 肽片段的质子磁共振研究。

• DOI: 10.1016/s0162-0134(00)80264-0
• 发表时间: 1982
• 期刊: Journal of inorganic biochemistry
• 影响因子: 3.9
• 作者: Leavis,PC;Evans,JS;Levine,BA
• 通讯作者: Levine,BA

Fluorescence lifetime and acrylamide quenching studies of the interactions between troponin subunits.

肌钙蛋白亚基之间相互作用的荧光寿命和丙烯酰胺猝灭研究。

• DOI: 10.1021/bi00313a023
• 发表时间: 1984
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Leavis,PC;Gowell,E;Tao,T
• 通讯作者: Tao,T

Quantitation of liquid-crystalline ordering in F-actin solutions.

• DOI: 10.1016/s0006-3495(92)81647-8
• 发表时间: 1992-09
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: Chris M. Coppin;Paul C. Leavis