Core B: Imaging/Marker Core

核心 B：成像/标记核心

• 批准号: 7876679
• 负责人: LAWRENCE E MATHES
• 金额: $ 22.32万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-21 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7876679
• 关键词: Animals; Cells; Comprehensive Cancer Center; Confocal Microscopy; Core Protein; Cytometry; Data Set; Disease; Environment; Flow Cytometry; Image; Image Analysis; Laboratories; Lead; Life; Lymphocyte Activation; MALDI-TOF Mass Spectrometry; Mass Spectrum Analysis; Measures; Methods; Microscope; Modeling; Nucleic Acids; Printing; Proteins; Publications; Research; Resources; Retroviridae; Sampling; Services; Staining method; Stains; System; Techniques; Technology; Time; Training; Virus; Work; base; cost effective; data acquisition; foot; imaging modality; in vivo; inhibitor/antagonist; instrument; instrumentation; novel; protein distribution; protein protein interaction; quality assurance; tumor

Core B of the PPG provides important support for all projects in standardized cell staining methods for flow cytometry and confocal microscopy, coordinates in vivo animal imaging, develops new imaging methods, and novel protein "foot printing" mass spectrometry methods. The new powerful protein foot-printing technology added to Core B services provides detailed information on protein-nucleic acid and proteinprotein interactions. The Imaging-Protein Core will provide common resources with quality assurance to the Five projects in the PPG. Multiuser instrumentation including the Leica Confocal Microscope and the MS in vivo Imaging System are used in a cost effective manner by trained technicians. Confocal imaging is an essential form of analysis for several key projects in the PPG. Live animal imaging using the MS instrumentation is rapidly becoming the standard for in vivo longitudinal Veal time' analysis of tumor distribution and virus expression and is essential for several of the proposed work in the competitive renewal. The AXIMA-CFR (MALDI-TOF) mass spectrometry is a key instrument to measure protein-protein, protein nucleic acid and protein-inhibitor, and has been added as a Core B service. Core B is within an outstanding environment and has documented interactions with all projects including numerous scientific publications. The Specific Aims of the Imaging-Protein Core B include: 1) To provide a standardized cell staining service of samples that will be analyzed by either confocal imaging through the PPG Imaging Core, or flow cytometry through the Analytical Cytometry Laboratory Shared Service of the Comprehensive Cancer Center, 2) To conduct standardized studies that makes use of Confocal Microscopy imaging for the quantitative analysis of virus and cell protein distribution and subcellular localization. To provide training and other techniques to the PPG user group, 3) To coordinate access to the MS Imaging System and provide training to the PPG user group, 4) To develop novel applications of the imaging instrumentation that will enhance the PPG directed research and lead more refined image analysis, 5) To employ the mass spectrometry (AXIMA-CFR MALDITOF) based protein foot printing technology for detailed characterization of protein-nucleic acid and proteinprotein interactions.

PPG的核心B为所有项目在标准化细胞染色方法方面提供了重要支持 细胞术和共聚焦显微镜，协调活体动物成像，开发新的成像方法， 和新的蛋白质“足迹”质谱学方法。新的强大的蛋白质足迹 添加到核心B服务的技术提供有关蛋白质-核酸和蛋白质的详细信息 互动。成像-蛋白质核心将提供具有质量保证的公共资源 PPG中的五个项目。多用户仪器，包括徕卡共焦显微镜和MS in VIVO成像系统由训练有素的技术人员以经济高效的方式使用。共聚焦成像是一种 PPG中几个关键项目的基本分析形式。使用MS进行活体动物成像 仪器设备正在迅速成为体内肿瘤纵向维氏时间分析的标准 分布和病毒表达，是竞争更新中的几项拟议工作所必需的。 AXIMA-CFR(MALDI-TOF)质谱仪是测定蛋白质、蛋白质、蛋白质的重要仪器。 核酸和蛋白质抑制剂，并已被添加为核心B服务。核心B在一个未完成的 环境方面，并记录了与包括许多科学出版物在内的所有项目的互动情况。 成像-蛋白质核心B的具体目标包括：1)提供标准化的细胞染色服务 将通过PPG成像核心进行共聚焦成像或通过流式细胞术进行分析的样本的 通过综合肿瘤中心分析细胞学实验室共享服务，2) 进行标准化研究，利用共聚焦显微镜成像进行定量分析 病毒和细胞蛋白的分布和亚细胞定位。提供培训和其他技术给 PPG用户组，3)协调对MS成像系统的访问并为PPG用户提供培训 小组，4)开发成像仪器的新应用，以增强PPG定向 研究和领导更精细的图像分析，5)使用质谱学(AXIMA-CFR MALDITOF) 基于蛋白质足迹技术的蛋白质-核酸和蛋白质的详细表征 互动。