AUTOMATED FINDING OF MODIFIED PEPTIDES FROM TANDEM MS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 利用PTM或氨基酸突变对多肽进行修饰对许多领域的研究具有重要意义。信号转导中的磷酸化和蛋白质损伤中的氧化都是例子。这些修饰会影响蛋白质的结构和活性，如果人工诱导，可以用来研究蛋白质的结构。在许多情况下，修饰的确切性质是未知的，或者有太多的修饰而不考虑使用蛋白质数据库搜索程序。编写了一个通用程序，用于发现任何类型的修改。它基于产物离子光谱与理论光谱或现有未修饰多肽光谱的2D相关性。使用用于相关性分析和定位修改位置的查看器。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Peptide modification by PTM or by mutation of amino acids is important to researcher in many fields. Phosphorilation in signaling and oxidation in protein damage are examples. These modifications affect protein structure and reactivity and can be used to study protein structures if induced artificially. In many cases the exact nature of the modification is not known or there are too many of them to consider using a protein database search program. A general program for discovering any kind of modification was written. It is based on 2D correlation of product-ion spectra to theoretical spectra or to existing spectra of unmodified peptides. A viewer for correlation analysis and locating the modification position is used.