MASS SPECTROMETRY SERVICE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 生物医学和生物有机质谱学中心为美国各地的学术客户提供质谱学服务。我们可以通过电子碰撞(EI)电离、化学电离(CI)、电喷雾电离(ESI)、快原子轰击(FAB退役)电离和基质辅助激光解吸电离(MALDI)来电离样品。我们拥有基于磁扇区、飞行时间、离子陷阱、Q-TOF、FT-ICR和FT-Orbitrap技术的分析仪。我们根据需要为客户提供标称(LR)、精确质量(HR，5ppm)和更高精度的质量(SHR，&lt；1ppm)光谱。我们还可以结合ESI提供LC/MS和LC/MS/MS服务。我们还拥有在蛋白质光谱中解卷积多电荷离子所需的计算工具。此外，我们提供几种仪器(用于MALDI板材分析的MALDI TOF/TOF和用于LR ESI的一台LCQ Deca)，并对用户进行无人操作培训，这些仪器是按使用小时计费的。 这为中心提供了对分子质量从小分子到几万道尔顿的样品进行质量分析的能力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Center for Biomedical and Bioorganic Mass Spectrometry provides mass spectral service for academic customers around the United States. We can ionize samples by electron-impact (EI) ionization, chemical ionization (CI), electrospray ionization (ESI), fast atom bombardment (FAB  retired) ionization, and matrix assisted laser desorption ionization (MALDI). We have analyzers based on magnetic sector, time-of-flight, ion trap, Q-TOF, FT-ICR, and FT-orbitrap technologies. We provide both nominal (LR), accurate mass (HR, 5ppm) and higher accurate mass (SHR, <1ppm) spectra to our customers as needed. We can also provide LC/MS and LC/MS/MS services in conjunction with ESI. We also have the computational tools needed to deconvolute the multiply charged ions in protein spectra. In addition, we make available and train users on a couple of instruments (MALDI TOF/TOF for MALDI plate analysis and one LCQ Deca for LR ESI) on a walk-up basis which we bill by the hours of usage. This provides the center with the capability to mass analyze samples with molecular weights ranging from small molecules all the way up to tens of kilodaltons.