CB: Tissue Resources and Molecular Pathology

CB：组织资源和分子病理学

• 批准号: 7921404
• 负责人: IGNACIO I. WISTUBA
• 金额: $ 25.84万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7921404
• 关键词: 3-Dimensional; Aliquot; Arts; Biological Markers; Blood specimen; Cancer Center; Cancer cell line; Cataloging; Catalogs; Cell Line; Cells; Clinical; Clinical Data; Collaborations; DNA; Data; Databases; Epithelial Cells; Funding; Human; Image; Image Analysis; Immunohistochemistry; In Situ; Institution; Lung; Malignant - descriptor; Malignant neoplasm of lung; Medical center; Methodology; Molecular; Non-Malignant; Paraffin; Pathologic; Pathologist; Pathology; Patients; Peer Review; Process; RNA; Reproduction spores; Research; Research Personnel; Resources; Site; Source; Specimen; Techniques; Texas; Tissue Microarray; Tissues; Translational Research; Tumor Cell Line; Universities; Xenograft procedure; anticancer research; base; c new; design; experience; innovation; molecular pathology; programs; tissue resource; tumor

SPORE Core B. Pathology and Tissue Resources Core. The Tissue Resources and Molecular Pathology Core will provide routine and innovative tissues and materials, as well as conventional and molecular pathology assistance, essential for achieving the aims of the SPORE projects. Routine materials include tumors and non-malignant lung specimens and tumor cell lines. Over 2,500 well-characterized tumors with annotated clinical data and 200 cell lines have been banked, and over 15,000 aliquots of tumor or cell line pellets, RNA or DNA or paraffin sections are available to SPORE investigators. State-of-art molecular pathology facilities devoted to lung cancer translational research directed by experienced lung cancer molecular pathologists are available. Our Aim 1 is to collect, process, store, catalog and distribute tissues, cells and blood specimens, both malignant and non-malignant, and relevant clinico-pathologic data, as requested by the various component projects of the SPORE program. Aim 2 is to develop and utilize innovative or routine tissue and cell resources that will aid in the successful completion of the SPORE program aims. Innovative materials include: a) cell pellets and tissue microarrays (TMAs) and high throughput image analysis of in situ techniques, b) new lung cancer cell lines and lung cancer xenografts made directly from patient specimens, c) new immortalized and non-immortalized human bronchial epithelial cells (HBECs) and 3-dimensional organotypic cultures. Aim 3 is to perform and interpret tissue-based molecular methodologies in close collaboration with the component projects of the SPORE program to satisfy their approved aims. This includes immunohistochemistry (IHC) information on over 90 lung cancer relevant biomarkers on the TMAs with information stored in an image database and integrated with the clinical annotations. Aim 4 is to promote collaboration among investigators in this SPORE, other lung cancer SPORES, other investigators in MD Anderson Cancer Center, UT Southwestern Medical Center, and other investigators nationally and internationally pursuing lung cancer relevant research. All of our five projects in this application will utilize CORE materials. Heavy utilization of our routine and innovative materials, and close interactions with the SPORE investigators will greatly aid the successful completion of the aims of our SPORE proposal. The SPORE Tissue Resource Core is designed and has operated as a facility receiving support from several other sources beyond SPORE funding including in a manner non-overlapping with other Cancer Center activities at both MD Anderson and UT Southwestern Medical Centers. This means that SPORE discoveries and data have far reaching beneficial effects across the range of lung cancer research conducted at these two institutions.

孢子核心B.病理学与组织资源核心。组织资源与分子 病理核心将提供常规和创新的组织和材料，以及常规和 分子病理学援助，对于实现孢子项目的目标至关重要。常规材料 包括肿瘤和非恶性肺标本和肿瘤细胞系。超过2500个特征良好的 有注释的临床数据和200个细胞系的肿瘤已经被储存，超过15,000个等量的肿瘤 或细胞系颗粒、RNA或DNA或石蜡切片可供孢子调查人员使用。最先进的 由经验丰富的肺癌专家指导的肺癌分子病理学设备 可以联系到癌症分子病理学家。我们的目标1是收集、加工、储存、编目和分发 恶性和非恶性的组织、细胞和血液标本以及相关的临床病理资料， 应孢子方案的各个组成部分项目的要求。目标2是开发和利用 创新的或常规的组织和细胞资源，有助于孢子的成功完成 计划的目的。创新材料包括：a)细胞小球和组织微阵列(TMA)和高通量 原位技术的图像分析，b)新的肺癌细胞系和肺癌移植瘤的制作 直接取自患者标本，c)新的永生化和非永生化的人支气管上皮细胞 (HBECs)和三维器官型培养。目标3是执行和解释基于组织的分子 与孢子方案组成部分项目密切合作的方法，以满足其 批准的目标。这包括90多个相关肺癌的免疫组织化学(IHC)信息 TMA上的生物标记物，信息存储在图像数据库中，并与临床集成 注释。目标4是促进这种孢子和其他肺癌的研究人员之间的合作 孢子，MD安德森癌症中心，德克萨斯大学西南医学中心的其他研究人员 国内外研究者致力于肺癌的相关研究。我们在中国的所有五个项目 这个应用程序将使用核心材料。大量使用我们的常规和创新材料，以及 与孢子调查人员的密切互动将极大地帮助我们成功地完成我们的目标 孢子求婚。孢子组织资源核心被设计并作为接收设施运行 除孢子基金以外的其他几个来源的支持，包括以不与 MD Anderson和德克萨斯大学西南医学中心的其他癌症中心活动。这意味着 孢子的发现和数据对各种肺癌都有深远的有益影响 在这两个机构进行的研究。