Computer/Electrical Engineering

计算机/电气工程

• 批准号: 7916613
• 负责人: Theodore Raphan
• 金额: $ 21.95万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7916613
• 关键词: 3-Dimensional; Algorithms; Animals; Back; Biological; Biological Models; Blood Pressure; Computer software; Computers; Data; Data Analyses; Data Files; Development; Devices; Dystonia; Electrical Engineering; Electronics; Equipment; Eye; Eye Movements; Force of Gravity; Goals; Head; Head Movements; Heart Rate; Human; Humidity; Image; Individual; Information Systems; Laboratories; Lasers; Lifting; Link; Linux; Locomotion; Maintenance; Maps; Measurement; Measures; Methodology; Microelectrodes; Modeling; Molecular; Molecular Biology; Molecular Models; Molecular Structure; Monkeys; Motion Sickness; Motor; Mus; Muscle; Nerve; Operating System; Patients; Physiological; Positioning Attribute; Predisposition; Rattus; Records; Reflex action; Relative (related person); Research; Research Personnel; Services; Side; Sledding; System; Techniques; Temperature; Testing; Time; Training; Universities; Update; Vestibular Nerve; Vestibule; Video Recording; Visual; Walking; Work; base; college; computer program; design; design and construction; meetings; member; micromanipulator; molecular dynamics; molecular modeling; multicore processor; open source; peroneal nerve; ranpirnase; research study; sample fixation; software development

Specific Aims: The purpose of the C/EE Core is to develop computer programs that control experimental equipment and analyze the data from the experiments of the P30 Core Center Vestibular Researchers and Molecular Biologists. The C/EE Core will also develop new analysis methodologies and design and construct new electronic equipment that makes collaborative and translational experiments possible. The Core is also responsible for updating computer software and providing a stable network. We propose two Specific Aims to meet the goals of the research: Specific Aim 1: Develop computer software and electronic hardware that will promote experiments across the Core Center, making effective use of cutting edge methodologies. These principles will be applied to the following projects (Primary collaborators listed for each set of tasks): A. Monkey Experiments (Yakushin/Raphan/Cohen): 1. Develop and install new positional controllers on the rotators using incremental recorders that have no dead zones. 2. Design control electronics for measuring the cervico-ocular reflex (COR) in monkeys. 3. Design and build (with the ME Core) new micromanipulators that can be used in adaptation experiments and for freely moving monkeys. 4. Modify the training system, previously developed for monkeys in convergence experiments, so that animals will watch a fixed point in space while they walk on a linear treadmill. 5. Develop a multiple channel video imaging system so that top, back and side images of the monkey can be viewed with the data while animals walk on linear or circular treadmills. This will also be used in human locomotion experiments. 6. Modify the eye movement recording system for monkey locomotion, which now records only one eye, to be binocular so the point of fixation in space can be determined. 7. Utilize the Kunin/Raphan algorithms developed for humans and for head-fixed monkeys on a linear sled to determine the binocular fixation point of the eyes in space during monkey locomotion (This will also be applied to all studies of human locomotion). B. Mouse Physiological, Neuroanatomic and Molecular Biological Studies (Yakushin/ Holstein/ Sealfon/Margolskee/Max/Bedrich/Cohen/Sclafani/Raphan): 1. Update the eye movement recording system for the mouse on the Cosmos rotator from Labview to Matlab to stabilize the frame rate and allow it to be modified. 2. Update the camera frame rates from 30 to 120 Hz. 3. Develop a multi-channel video imaging capability to store binocular eye images in conjunction with the horizontal, torsional and vertical eye position data that are transformed from the video images. 4. Generate analysis techniques for establishing eye movements relative to head coordinates of the mouse. 5. Generate apparatus for heart rate and blood pressure measurements in the rat to determine the impact of the vestibule-sympathetic reflex during changes in head position relative to gravity. 6. Utilize software and hardware developed for humans to stimulate the vestibular nerves of rats and activate the vestibulo-sympathetic reflex. C. Molecular Biology Model Implementation (Margolskee/Max/Mossinger/Raphan): Design and implement multiprocessor workstations to facilitate individual use of computers in molecular biological modeling. Update the molecular modeling software so that open source systems such as Visual Molecular Dynamics (VMD) can be utilized under Linux. This would make analysis of molecular structure more accessible to individual investigators. D. Human Motion Sickness and Vestibulo-Autonomic Studies (Dai/Cohen/Kaufmann/Raphan/ Straumann): 1. Convert the data system of the University of Zurich (for Dr. Straumann, who is collaborating on the motion sickness project), into a format compatible with the VMF data analysis system used at Mount Sinai. 2. Implement the video-based binocular recording technique developed for the human locomotion project in the OVAR rotator. 3. Upgrade the controller on the circular treadmill so that it can be used to adapt the vertical aVOR time constant to determine if it reduces motion sickness susceptibility. 4. Develop a miniature device that will be placed on the rotating chair to record muscle sympathetic nerve activity (MSNA) from the peroneal nerve during OVAR; also develop a portable stimulator to map the trajectory of the peroneal nerve to determine the point of insertion of the microelectrodes; both devices must be small, portable and battery powered. 5. With the ME Core, build a new 3-D optokinetic controller and stimulator for the OVAR enclosure with new gears and motors. 6. Build a computer-controlled device to measure the subjective visual vertical when stationary and during OVAR. 7. Work with the ME Core to establish humidity and temperature control in the OVAR enclosure. E. Human Locomotion Studies (Raphan/Cohen/Cho/Smouha/Olanow): 1. With the ME Core, devise a controller to lift the back of the linear treadmill to test subjects for downhill walking. 2. Build a video system that records the top, side and back while walking; embed the video into the data files. 3. Modify the control circuits of the linear and circular treadmills to be computer controlled. 4. Devise a control mechanism to present visual laser targets in random order for studies of head movement control in normals and patients with dystonia. Specific Aim 2: Do software and hardware maintenance. Provide support services that will maintain and upgrade existing hardware and software and work to establish links between the new members of the Research Base and the extant Core Members. Assist in building and interfacing the hardware developed in Specific Aim 1 and maintain network connections across the Core both at Brooklyn College and Mount Sinai. Also install and maintain new operating systems and applications software to keep the laboratories at the cutting edge of technological development and troubleshoot problems as they arise.

具体目标：C/EE 核心的目的是开发控制实验的计算机程序 设备并分析 P30 核心中心前庭研究人员的实验数据 分子生物学家。 C/EE 核心还将开发新的分析方法以及设计和构建 新的电子设备使协作和转化实验成为可能。核心也是 负责更新计算机软件并提供稳定的网络。我们提出两个具体目标 达到研究目标： 具体目标1：开发促进实验的计算机软件和电子硬件 整个核心中心，有效利用尖端方法。这些原则将 适用于以下项目（每组任务列出主要合作者）： A. 猴子实验（Yakushin/Raphan/Cohen）： 1. 开发并安装新的位置控制器 使用无死区的增量记录器的旋转器。 2. 设计用于测量的控制电子设备 猴子的颈眼反射（COR）。 3. 设计和构建（使用 ME Core）新的微操纵器 可用于适应实验和自由移动的猴子。 4.修改培训体系， 以前为猴子开发的收敛实验，这样动物就会观察一个固定点 他们在线性跑步机上行走时的空间。 5. 开发多通道视频成像系统，以便顶部、 当动物沿着直线或圆形行走时，可以通过数据查看猴子的背部和侧面图像 跑步机。这也将用于人体运动实验。 6.修改眼动记录 猴子运动系统现在只记录一只眼睛，变成了双眼，所以注视点 空间可以确定。 7. 利用为人类和头部固定开发的 Kunin/Raphan 算法 猴子在线性雪橇上确定猴子在空间中的双眼注视点 运动（这也将应用于所有人类运动的研究）。 B. 小鼠生理学、神经解剖学和分子生物学研究（Yakushin/ Holstein/ Sealfon/Margolskee/Max/Bedrich/Cohen/Sclafani/Raphan)：1.更新眼动记录系统 对于Cosmos旋转器上的鼠标从Labview到Matlab以稳定帧速率并允许其 修改的。 2. 将相机帧速率从 30 Hz 更新为 120 Hz。 3. 开发多路视频成像 能够存储双眼图像以及水平、扭转和垂直眼睛位置 由视频图像转换而来的数据。 4. 生成建立眼睛的分析技术 相对于鼠标头部坐标的移动。 5. 生成心率和血液的装置 测量大鼠的压力以确定变化期间前庭交感神经反射的影响 相对于重力的头部位置。 6.利用为人类开发的软件和硬件来刺激 大鼠前庭神经并激活前庭交感反射。 C. 分子生物学模型实现（Margolskee/Max/Mossinger/Raphan）：设计和 实施多处理器工作站以方便个人在分子生物学中使用计算机 造型。更新分子建模软件，使 Visual Molecular 等开源系统 Dynamics (VMD) 可以在 Linux 下使用。这将使分子结构分析变得更容易 给个别调查员。 D. 人类晕动病和前庭自主神经研究 (Dai/Cohen/Kaufmann/Raphan/ Straumann）：1.转换苏黎世大学的数据系统（为Straumann博士，他正在合作 晕动病项目），转换为与西奈山使用的 VMF 数据分析系统兼容的格式。 2. 实施为人类运动项目开发的基于视频的双目记录技术 OVAR 旋转器。 3、升级圆形跑步机上的控制器，使其可以适应垂直方向 aVOR 时间常数以确定它是否降低晕动病的易感性。 4. 开发微型装置 将放置在旋转椅上以记录腓骨的肌肉交感神经活动 (MSNA) OVAR期间的神经；还开发了一种便携式刺激器来绘制腓神经的轨迹 确定微电极的插入点；两种设备都必须小巧、便携且有电池 供电。 5. 使用 ME Core，为 OVAR 外壳构建新的 3-D 光动控制器和刺激器 配备新的齿轮和电机。 6. 构建计算机控制的主观视觉垂直测量装置 静止时和 OVAR 期间。 7. 与 ME Core 合作建立湿度和温度控制 OVAR 外壳。 E. 人体运动研究 (Raphan/Cohen/Cho/Smouha/Olanow)： 1. 使用 ME 核心，设计一个 控制器抬起线性跑步机的后部以测试受试者下坡行走。 2. 构建一个视频系统 记录行走时的顶部、侧面和背部；将视频嵌入到数据文件中。 3.修改控制电路 计算机控制的线性和圆形跑步机。 4. 设计一种控制机制来呈现视觉效果 随机排列的激光目标，用于研究正常人和肌张力障碍患者的头部运动控制。 具体目标2：做好软硬件维护。提供支持服务，以维护和 升级现有的硬件和软件，并努力在新成员之间建立联系 研究基地及现有核心成员。协助构建和连接开发的硬件 具体目标 1 维持布鲁克林学院和西奈山核心网络的网络连接。 还安装和维护新的操作系统和应用软件，使实验室保持在最佳状态 掌握最前沿的技术发展并解决出现的问题。