Computer/Electrical Engineering

具体目标：C/EE核心的目的是开发控制实验的计算机程序 设备和分析来自P30核心中心前庭研究人员和 分子生物学家。C/EE核心还将开发新的分析方法以及设计和建造 新的电子设备，使协作和转换实验成为可能。核心也是 负责更新计算机软件，提供稳定的网络。我们提出了两个具体目标来 达到研究目标： 具体目标1：开发将促进实验的计算机软件和电子硬件 在整个核心中心，有效地利用尖端方法。这些原则将是 适用于以下项目(为每组任务列出的主要协作者)： A.猴子实验(雅库辛/拉班/科恩)：1.开发和安装新的位置控制器 旋转器使用无死区的增量式记录器。2.设计用于测量的控制电子设备 猴子的颈眼反射(COR)。3.设计和制造(使用ME核心)新的微操作器， 可用于适应实验和自由活动的猴子。4.修改培训体系， 以前为猴子开发的收敛实验，让动物在定点观看 当他们在直线跑步机上行走时，他们可以在空间中行走。5.开发多通道视频成像系统，使顶部， 当动物在直线或圆形上行走时，可以使用数据查看猴子的背部和侧面图像 跑步机。这也将用于人类的运动实验。6.修改眼动记录 猴子移动系统，现在只记录一只眼睛，是双目的，所以凝视点在 空间是可以确定的。7.利用为人类和头部固定器开发的库宁/拉班算法 在直线雪橇上测定猴眼在太空中的双眼注视点 运动(这也将应用于对人类运动的所有研究)。 B.小鼠生理、神经解剖学和分子生物学研究(雅库辛/荷斯坦/ Sealfon/Margolskee/Max/Bedrich/Cohen/Sclafani/Raphan)：1.更新眼动记录系统 将鼠标放在Cosmos旋转器上，从LabVIEW到MatLab来稳定帧速率，并允许它 修改过的。2.将摄像头的帧率从30赫兹更新到120赫兹。3.开发多通道视频成像 能够结合水平、扭转和垂直眼睛位置存储双目图像 从视频图像转换而来的数据。4.生成用于建立眼睛的分析技术 相对于鼠标头部坐标的移动。5.生成心率和血流仪 测量大鼠压力以确定变化过程中前庭-交感反射的影响 头部相对于重力的位置。6.利用为人类开发的软件和硬件来刺激 刺激大鼠前庭神经，激活前庭交感神经反射。 C.分子生物学模型的实施(马戈尔斯基/马克斯/莫辛格/拉潘)：设计和 实施多处理器工作站，促进计算机在分子生物学领域的个人使用 模特儿。更新分子建模软件，以便开放源码系统，如可视化分子 动态(VMD)可以在Linux下使用。这将使分析分子结构变得更容易 给个人调查员。 D.人类运动病和前庭自主研究(Dai/Cohen/Kaufmann/Raphan/ 斯图尔特兰)：1.转换苏黎世大学的数据系统(为斯图尔特兰博士，他正在 晕动病项目)，转换成与西奈山使用的VMF数据分析系统兼容的格式。 2.将为人类运动项目开发的基于视频的双目记录技术在 OVAR旋转器。3.升级圆形跑步机上的控制器，使其可以用于适应垂直方向 Avor时间常数以确定它是否可以降低晕动病的敏感性。4.研制微型设备 这将被放置在转椅上，以记录来自腓骨的肌肉交感神经活动(MSNA) OVAR过程中的神经；还开发了一种便携式刺激器，以映射腓神经的轨迹 确定微电极的插入点；两个设备都必须是小型、便携和带电池的 动力十足。5.使用ME核心，为OVAR外壳构建新的3-D视动控制器和刺激器 配备新的齿轮和马达。6.建立一个计算机控制的装置来测量主观视觉垂直 当静止时和在OVAR期间。7.与ME Core合作建立湿度和温度控制 OVAR外壳。 E.人类运动研究(Raphan/Cohen/Cho/Smuha/Olanow)：1.利用ME核心，设计一个 控制器抬起线性跑步机的后部，以测试下坡行走的受试者。2.构建视频系统， 记录行走时的顶部、侧面和背面；将视频嵌入到数据文件中。3.修改控制电路 直线跑步机和圆形跑步机将由计算机控制。4.设计一种控制机制来呈现视觉 随机顺序激光靶标用于研究正常人和肌张力障碍患者的头部运动控制。 具体目标二：做好软硬件维护。提供支持服务，以维护和 升级现有硬件和软件，并努力在新成员之间建立联系 研究基地和现有的核心成员。协助构建和连接中开发的硬件 具体目标1，并保持布鲁克林学院和西奈山核心地区的网络连接。 还要安装和维护新的操作系统和应用程序软件，使实验室保持在 技术发展的前沿，并在出现问题时进行故障排除。

Specific Aims: The purpose of the C/EE Core is to develop computer programs that control experimental equipment and analyze the data from the experiments of the P30 Core Center Vestibular Researchers and Molecular Biologists. The C/EE Core will also develop new analysis methodologies and design and construct new electronic equipment that makes collaborative and translational experiments possible. The Core is also responsible for updating computer software and providing a stable network. We propose two Specific Aims to meet the goals of the research: Specific Aim 1: Develop computer software and electronic hardware that will promote experiments across the Core Center, making effective use of cutting edge methodologies. These principles will be applied to the following projects (Primary collaborators listed for each set of tasks): A. Monkey Experiments (Yakushin/Raphan/Cohen): 1. Develop and install new positional controllers on the rotators using incremental recorders that have no dead zones. 2. Design control electronics for measuring the cervico-ocular reflex (COR) in monkeys. 3. Design and build (with the ME Core) new micromanipulators that can be used in adaptation experiments and for freely moving monkeys. 4. Modify the training system, previously developed for monkeys in convergence experiments, so that animals will watch a fixed point in space while they walk on a linear treadmill. 5. Develop a multiple channel video imaging system so that top, back and side images of the monkey can be viewed with the data while animals walk on linear or circular treadmills. This will also be used in human locomotion experiments. 6. Modify the eye movement recording system for monkey locomotion, which now records only one eye, to be binocular so the point of fixation in space can be determined. 7. Utilize the Kunin/Raphan algorithms developed for humans and for head-fixed monkeys on a linear sled to determine the binocular fixation point of the eyes in space during monkey locomotion (This will also be applied to all studies of human locomotion). B. Mouse Physiological, Neuroanatomic and Molecular Biological Studies (Yakushin/ Holstein/ Sealfon/Margolskee/Max/Bedrich/Cohen/Sclafani/Raphan): 1. Update the eye movement recording system for the mouse on the Cosmos rotator from Labview to Matlab to stabilize the frame rate and allow it to be modified. 2. Update the camera frame rates from 30 to 120 Hz. 3. Develop a multi-channel video imaging capability to store binocular eye images in conjunction with the horizontal, torsional and vertical eye position data that are transformed from the video images. 4. Generate analysis techniques for establishing eye movements relative to head coordinates of the mouse. 5. Generate apparatus for heart rate and blood pressure measurements in the rat to determine the impact of the vestibule-sympathetic reflex during changes in head position relative to gravity. 6. Utilize software and hardware developed for humans to stimulate the vestibular nerves of rats and activate the vestibulo-sympathetic reflex. C. Molecular Biology Model Implementation (Margolskee/Max/Mossinger/Raphan): Design and implement multiprocessor workstations to facilitate individual use of computers in molecular biological modeling. Update the molecular modeling software so that open source systems such as Visual Molecular Dynamics (VMD) can be utilized under Linux. This would make analysis of molecular structure more accessible to individual investigators. D. Human Motion Sickness and Vestibulo-Autonomic Studies (Dai/Cohen/Kaufmann/Raphan/ Straumann): 1. Convert the data system of the University of Zurich (for Dr. Straumann, who is collaborating on the motion sickness project), into a format compatible with the VMF data analysis system used at Mount Sinai. 2. Implement the video-based binocular recording technique developed for the human locomotion project in the OVAR rotator. 3. Upgrade the controller on the circular treadmill so that it can be used to adapt the vertical aVOR time constant to determine if it reduces motion sickness susceptibility. 4. Develop a miniature device that will be placed on the rotating chair to record muscle sympathetic nerve activity (MSNA) from the peroneal nerve during OVAR; also develop a portable stimulator to map the trajectory of the peroneal nerve to determine the point of insertion of the microelectrodes; both devices must be small, portable and battery powered. 5. With the ME Core, build a new 3-D optokinetic controller and stimulator for the OVAR enclosure with new gears and motors. 6. Build a computer-controlled device to measure the subjective visual vertical when stationary and during OVAR. 7. Work with the ME Core to establish humidity and temperature control in the OVAR enclosure. E. Human Locomotion Studies (Raphan/Cohen/Cho/Smouha/Olanow): 1. With the ME Core, devise a controller to lift the back of the linear treadmill to test subjects for downhill walking. 2. Build a video system that records the top, side and back while walking; embed the video into the data files. 3. Modify the control circuits of the linear and circular treadmills to be computer controlled. 4. Devise a control mechanism to present visual laser targets in random order for studies of head movement control in normals and patients with dystonia. Specific Aim 2: Do software and hardware maintenance. Provide support services that will maintain and upgrade existing hardware and software and work to establish links between the new members of the Research Base and the extant Core Members. Assist in building and interfacing the hardware developed in Specific Aim 1 and maintain network connections across the Core both at Brooklyn College and Mount Sinai. Also install and maintain new operating systems and applications software to keep the laboratories at the cutting edge of technological development and troubleshoot problems as they arise.