PLASMA FFA ELEVATION ON FOREARM BLOOD FLOW AND CAP RECRUITMENT AFTER INSULIN

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 最近的证据表明，生理剂量的胰岛素输注诱导骨骼肌血管舒张，包括新血管的募集。血浆脂肪酸升高后对胰岛素血管效应的抵抗可能导致2型糖尿病、肥胖和高血压中观察到的代谢紊乱。 我们将研究22名瘦健康成人的总血流量、前臂毛细血管募集和全身葡萄糖利用率，分别在基线和两个不同的场合进行两小时的正葡萄糖胰岛素钳夹。 在一个钳夹中，我们将输注“Intradherid”和肝素，以提高脂肪酸水平，另一个钳夹将在不输注“Intradherid”/肝素的情况下进行，作为对照。 我们将使用多普勒超声和CEU（微泡造影剂增强超声）测量血流量和毛细血管募集。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recent evidence suggests that an insulin infusion at a physiologic dose induces skeletal muscle vasodilation, including the recruitment of new blood vessels. Resistance to insulin's vascular effects after elevation of plasma fatty acids may contribute to the metabolic derangements seen in Type 2 DM, obesity and hypertension. We will study total blood flow, capillary recruitment in the forearm, and whole body glucose utilization of 22 lean healthy adults at baseline and after a two hour euglycemic insulin clamp on two separate occasions. With one clamp, we will infuse "Intralipid" and heparin creating a fatty acid elevation, and the other clamp will be conducted without the "Intralipid"/heparin infusion, as a control. We will measure the blood flow and capillary recruitment using Doppler Ultrasound and CEU (microbubble contrast-enhanced ultrasound).