PLASMA FFA ELEVATION ON FOREARM BLOOD FLOW AND CAP RECRUITMENT AFTER INSULIN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 最近的证据表明，生理剂量的胰岛素输注可以诱导骨骼肌血管扩张，包括新血管的募集。血浆脂肪酸升高后对胰岛素血管效应的抵抗可能会导致2型糖尿病、肥胖症和高血压的代谢紊乱。我们将研究22名瘦削的健康成年人在基线和两次正常血糖胰岛素钳夹两小时后的总血流量、前臂毛细血管募集和全身葡萄糖利用。其中一个钳子将注入“Intralipid”和肝素产生脂肪酸升高，另一个钳子将不注入“Intralipid”/肝素作为对照。我们将使用多普勒超声和CEU(微泡超声造影)测量血流和毛细血管募集。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recent evidence suggests that an insulin infusion at a physiologic dose induces skeletal muscle vasodilation, including the recruitment of new blood vessels. Resistance to insulin's vascular effects after elevation of plasma fatty acids may contribute to the metabolic derangements seen in Type 2 DM, obesity and hypertension. We will study total blood flow, capillary recruitment in the forearm, and whole body glucose utilization of 22 lean healthy adults at baseline and after a two hour euglycemic insulin clamp on two separate occasions. With one clamp, we will infuse "Intralipid" and heparin creating a fatty acid elevation, and the other clamp will be conducted without the "Intralipid"/heparin infusion, as a control. We will measure the blood flow and capillary recruitment using Doppler Ultrasound and CEU (microbubble contrast-enhanced ultrasound).