EFFECT OF ELEVATING PLASMA FFA ON FOREARM BLOOD FLOW AND CAPILLARY RECRUITMENT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 最近的证据表明，混合餐可以诱导骨骼肌血管扩张，包括新血管的募集。这种扩张可能是由于餐后胰岛素分泌增加所致。血浆脂肪酸升高后对胰岛素血管效应的抵抗可能会导致2型糖尿病、肥胖症和高血压的代谢紊乱。目前，测量人类骨骼肌中微血管体积的方法是弗吉尼亚大学独有的。在我们的方案中，我们将研究22名瘦成人在测量的混合餐前后、接受Intralipid/肝素和一次不输液的总血流量和前臂毛细血管募集以及全身葡萄糖利用。我们将使用多普勒U/S和微泡超声造影进行这些测量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recent evidence suggests that a mixed meal induces skeletal muscle vasodilation, including the recruitment of new blood vessels. This dilation is probably a result of increased insulin secretion after the meal. Resistance to insulin's vascular effects after elevation of plasma fatty acids may contribute to the metabolic derangements seen in Type 2 DM, obesity and hypertension. The method of measurement of microvascular volume in the skeletal muscle of humans is currently unique to the University of Virginia. In our protocol, we will study total blood flow and capillary recruitment in the forearm, and whole body glucose utilization of 22 lean adults before and after a measured mixed meal, once receiving an Intralipid/heparin, and once without the infusion. We will make these measurements using Doppler U/S and microbubble contrast-enhanced ultrasound.