GENOTYPIC AND PHENOTYPIC RESPONSE OF BREAST EPITHELIAL CELLS FROM WOMEN

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 项目目的： I. 招募接受乳房缩小术的女性，提供流行病学资料、血液、尿液和乳房组织，作为数据和组织储存库。 2. 利用该库研究低风险基因，调查基因-环境相互作用，并建立涉及致癌物代谢，DNA损伤和对DNA损伤的反应的基因型-表型关系，以识别或验证癌症风险的中间生物标志物的使用。 3. 确定替代组织（如血液、口腔拭子和尿液）中测量的生物标志物与乳腺组织（研究致癌物的靶器官）中相同生物标志物水平的关系。 提供本项目剩余部分的时间表和工作计划： 三年来，建立培养物，测定P53和细胞凋亡对DNA损伤的反应，然后确定负责这种反应的基因。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Purpose of project: I. Recruit women who are undergoing reduction mamoplasty to provide an epidemiological profile, blood, urine, and breast tissue, which will be established as a data and tissue repository. 2. Utilize the repository to study low penetrance genes, investigate gene-environment interactions and establish genotype-phenotype relationships involving carcinogen metabolism, DNA damage, and response to DNA damage, in order to identify or validate the use of intermediate biomarkers of cancer risk. 3. To identify the relationship of biomarkers measured in surrogate tissues such as blood, buccal swabs and urine to the level of the same biomarker in the breast tissue, which is the target organ for the carcinogens under study. PROVIDE TIME LINE AND WORK PLANNED FOR THE REMAINDER OF THIS PROJECT: THREE YEARS, ESTABLISH CULTURES, DETERMINE P53 AND APOPTOSIS RESPONSE TO DNA DAMAGE, AND THEN IDENTIFY THE GENES RESPONSIBLE FOR SUCH