GENOTYPIC AND PHENOTYPIC RESPONSE OF BREAST EPITHELIAL CELLS FROM WOMEN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 项目目的： 招募正在接受乳房缩小整形手术的妇女，以提供流行病学概况、血液、尿液和乳房组织，这些将被建立为数据和组织储存库。 2.利用该库研究低外显性基因，研究基因与环境的相互作用，建立涉及致癌物质代谢、DNA损伤和DNA损伤反应的基因-表型关系，以识别或验证癌症风险的中间生物标志物的使用。 3.确定血液、口腔拭子和尿液等替代组织中测定的生物标志物与乳房组织中相同生物标志物水平的关系，乳房组织是所研究致癌物的靶器官。 提供该项目剩余部分的时间表和计划工作： 三年，建立培养物，确定p53和细胞凋亡对DNA损伤的反应，然后确定与此相关的基因

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Purpose of project: I. Recruit women who are undergoing reduction mamoplasty to provide an epidemiological profile, blood, urine, and breast tissue, which will be established as a data and tissue repository. 2. Utilize the repository to study low penetrance genes, investigate gene-environment interactions and establish genotype-phenotype relationships involving carcinogen metabolism, DNA damage, and response to DNA damage, in order to identify or validate the use of intermediate biomarkers of cancer risk. 3. To identify the relationship of biomarkers measured in surrogate tissues such as blood, buccal swabs and urine to the level of the same biomarker in the breast tissue, which is the target organ for the carcinogens under study. PROVIDE TIME LINE AND WORK PLANNED FOR THE REMAINDER OF THIS PROJECT: THREE YEARS, ESTABLISH CULTURES, DETERMINE P53 AND APOPTOSIS RESPONSE TO DNA DAMAGE, AND THEN IDENTIFY THE GENES RESPONSIBLE FOR SUCH