STRUCTURE-FUNCTION RELATIONSHIPS IN FE- AND CO-CONTAINING NITRILE HYDRATASES

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 本研究的总体目标是利用X射线吸收光谱分析腈水合酶（NHases）的反应机理。该项目的重要性因腈在工业上的广泛应用而凸显出来，腈可用于生产大量含有胺、酰胺、脒、羧酸、酯、醛、酮和杂环化合物的特种化学品。这些化合物在广泛的化学反应中用作生产溶剂、萃取剂、药物、农药和聚合物的原料。拟开展的研究旨在确定来自嗜热假诺卡氏菌JCM 3095的Co型腈水合酶（PtNHase）和来自睾丸酮丛毛单胞菌的Fe型腈水合酶（CtNHase）的反应机理。这项工作的成功完成不仅将深入了解腈水合酶的催化机制，还将为腈水合酶在有机化学加工、工业合成和生物修复工具中的应用提供新的见解。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The overall goal of this research is to use x-ray absorption spectroscopy to analyze the reaction mechanism of nitrile hydratases (NHases). The importance of this project is underscored by the extensive industrial use of nitriles to produce a broad number of specialty chemicals containing amines, amides, amidines, carboxylic acids, esters, aldehydes, ketones, and heterocyclic compounds. These compounds are used in a wide array of chemical reactions as feedstock for the production of solvents, extractants, pharmaceuticals, pesticides, and polymers. The proposed research aims to define the reaction mechanisms for both the Co-type nitrile hydratase from Pseudonocardia thermophila JCM 3095 (PtNHase) and the Fe-type NHase from Comamonas testosteroni (CtNHase). The successful completion of the proposed work will not only provide insight into the catalytic mechanism of NHase enzymes, but will also provide new insight into the use of NHase enzymes in organic chemical processing, industrial syntheses, and bioremediation tools.