STRUCTURE-FUNCTION RELATIONSHIPS IN FE- AND CO-CONTAINING NITRILE HYDRATASES

含铁和共基腈水合酶的结构-功能关系

• 批准号: 8170199
• 负责人: ROBERT A SCOTT
• 金额: $ 0.07万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170199
• 关键词: Aldehydes; Amides; Amidines; Amines; Bioremediations; Carboxylic Acids; Chemicals; Comamonas testosteroni; Computer Retrieval of Information on Scientific Projects Database; Enzymes; Esters; Funding; Goals; Grant; Heterocyclic Compounds; Institution; Ketones; Nitriles; Organic Chemicals; Pesticides; Pharmacologic Substance; Polymers; Process; Production; Reaction; Research; Research Personnel; Resources; Solvents; Source; Spectrum Analysis; Structure-Activity Relationship; United States National Institutes of Health; Work; absorption; chemical reaction; insight; medical specialties; nitrile hydratase; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The overall goal of this research is to use x-ray absorption spectroscopy to analyze the reaction mechanism of nitrile hydratases (NHases). The importance of this project is underscored by the extensive industrial use of nitriles to produce a broad number of specialty chemicals containing amines, amides, amidines, carboxylic acids, esters, aldehydes, ketones, and heterocyclic compounds. These compounds are used in a wide array of chemical reactions as feedstock for the production of solvents, extractants, pharmaceuticals, pesticides, and polymers. The proposed research aims to define the reaction mechanisms for both the Co-type nitrile hydratase from Pseudonocardia thermophila JCM 3095 (PtNHase) and the Fe-type NHase from Comamonas testosteroni (CtNHase). The successful completion of the proposed work will not only provide insight into the catalytic mechanism of NHase enzymes, but will also provide new insight into the use of NHase enzymes in organic chemical processing, industrial syntheses, and bioremediation tools.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 这项研究的总体目标是使用X射线吸收光谱来分析硝酸水合物（NHASE）的反应机理。该项目的重要性强调了硝酸盐的广泛工业用途来生产大量含有胺，酰胺，胺，羧酸，酯，醛，醛，酮，酮和杂环化合物的特种化学品。这些化合物用于多种化学反应中，作为用于产生溶剂，提取剂，药物，农药和聚合物的原料。拟议的研究旨在定义嗜热心动过速JCM 3095（PTNHASE）和Comamonas testosteroni（CTNHASE）的Fe-type NHase（ctnhase）的Fe-type NHase的反应机制。拟议工作的成功完成不仅将提供对NHASE酶催化机制的见解，而且还将为使用NHASE酶在有机化学化学加工，工业合成和生物治疗工具中的使用提供新的见解。