HIGH-THROUGHPUT X-RAY ABSORPTION SPECTROSCOPY FOR METALLOPROTEOMICS

用于金属蛋白质组学的高通量 X 射线吸收光谱

• 批准号: 7954213
• 负责人: ROBERT A SCOTT
• 金额: $ 0.52万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954213
• 关键词: Address; Biological; Complement; Computer Retrieval of Information on Scientific Projects Database; Development; Ecology; Environment; Feasibility Studies; Funding; Grant; Growth; Heavy Metals; Institution; Metals; Microarray Analysis; Modeling; Proteins; Proteome; Pyrococcus furiosus; Ralstonia; Research; Research Personnel; Resources; Roentgen Rays; Science; Shewanella; Site; Source; Spectrum Analysis; Stress; Technology; Testing; United States National Institutes of Health; absorption; base; beamline; design; functional genomics; member; microbial; microorganism; new technology; structural biology; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to partner with SSRL SMB beamline development efforts to provide a new technology, called high-throughput x-ray absorption spectroscopy (HTXAS), designed to provide a metal-based view of the proteome. Whereas functional genomics (microarray) technology interrogates the expression levels of the members of the proteome, HTXAS will provide metal-site structural information about members of the metalloproteome. These technologies address biological questions about the effect of environmental stress on expression of certain proteins and on assembly of certain metal sites in proteins, respectively. As one example of this discovery science approach, we propose to apply it to questions of microbial ecology. In particular, how does a microorganism's metalloproteome complement change under the stress of heavy metals in its growth environment? Toward that end, we are using Pyrococcus furiosus as a model and Shewanella oneidensis and Ralstonia metallidurans as our first targets. The requested beamtime is for feasibility studies of this technology and testing beamline development activities associated with creating this technology. Spinoffs of this technological development are expected to have a major impact on "standard" biological XAS studies also.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们建议与 SSRL SMB 光束线开发团队合作，提供一种称为高通量 X 射线吸收光谱 (HTXAS) 的新技术，旨在提供基于金属的蛋白质组视图。功能基因组学（微阵列）技术询问蛋白质组成员的表达水平，而 HTXAS 将提供有关金属蛋白质组成员的金属位点结构信息。这些技术分别解决了环境压力对某些蛋白质表达和蛋白质中某些金属位点组装的影响的生物学问题。作为这种发现科学方法的一个例子，我们建议将其应用于微生物生态学问题。特别是，微生物的金属蛋白质组补体在其生长环境的重金属胁迫下如何变化？为此，我们使用激烈火球菌作为模型，并使用希瓦氏菌和金属拉尔斯顿菌作为我们的首要目标。请求的射束时间用于该技术的可行性研究以及测试与创建该技术相关的射束线开发活动。这项技术发展的副产品预计也会对“标准”生物 XAS 研究产生重大影响。