FIBER OPTIC/PHOTOMULTIPLIER TUBE (PMT) INTERFACE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目是正在进行的努力的一部分，将我们的电化学传感器技术与光子成像化学传感技术相结合。为了做到这一点，我们已经建立了一个光子计数站，作为一个照度计和收集荧光信号，通过一个拉光纤。Hamamatsu R464光电倍增管用于光子检测。National instruments PCI-6036 E用于计数事件并控制uniblitz vs-25快门。软件界面使用Lab-View 7构建。Uniblitz快门驱动器利用Microchip PIC 16 F628微控制器脉宽调制器驱动升压转换器，通过单个22 uh电感器将5 V转换为70 V。Uniblitz驱动电路是压电管控制精密电路的基础。Pic 16 F628芯片上还有一个16位计数器，我们计划使用它来消除PCI-6036 e，使设备可以从单个RS232端口操作。利用这种光子计数系统，我们可以探索位于细胞和边界层中的发光化学传感器和弱荧光信号的使用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is part of an ongoing effort to integrate our electrochemical sensor technologies with opportunitites in chemical sensing by photon imaging. To do this we have constructed a photon counting station for use as an illuminometer and collection of fluorescence signals through a pulled fiber optic. A Hamamatsu R464 photo-multiplier tube is used for photon detection. A National instruments PCI-6036E is used to count the events and control a uniblitz vs-25 shutter. The software interface has been constructed using Lab-View 7. The Uniblitz shutter drive utilizes a Microchip PIC16F628 micro-controller pulse width modulator to drive a boost converter, converting 5 volts to 70 volts with a single 22uh inductor. The Uniblitz driver circuit serves as the basis for a precision circuit for piezo tube control. The Pic16F628 also has on the chip a 16 bit counter, we plan to use this to eliminate the PCI-6036e allowing the device to operate from a single RS232 port. With this photon counting system we can explore the use of luminescent chemical sensors and weak florescent signals located in cells and in the boundary layers.