FIBER OPTIC/PHOTOMULTIPLIER TUBE (PMT) INTERFACE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目是将我们的电化学传感器技术与光子成像化学传感机会相结合的持续努力的一部分。为了做到这一点，我们建造了一个光子计数站，用作照度计，并通过拉出的光纤收集荧光信号。使用滨松R464光电倍增管进行光子探测。国家仪器公司的PCI-6036E被用来对事件进行计数，并控制Unblitz VS-25快门。软件界面使用Lab-View 7构建。Uniblitz快门驱动器使用微芯片PIC16F628微控制器脉宽调制器来驱动升压转换器，用一个22eh电感将5伏转换到70伏。Uniblitz驱动电路是用于压电管控制的精密电路的基础。Pic16F628还在芯片上有一个16位计数器，我们计划使用它来消除允许设备从单个RS232端口操作的PCI-6036e。利用这个光子计数系统，我们可以探索发光化学传感器和位于细胞和边界层中的微弱荧光信号的使用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project is part of an ongoing effort to integrate our electrochemical sensor technologies with opportunitites in chemical sensing by photon imaging. To do this we have constructed a photon counting station for use as an illuminometer and collection of fluorescence signals through a pulled fiber optic. A Hamamatsu R464 photo-multiplier tube is used for photon detection. A National instruments PCI-6036E is used to count the events and control a uniblitz vs-25 shutter. The software interface has been constructed using Lab-View 7. The Uniblitz shutter drive utilizes a Microchip PIC16F628 micro-controller pulse width modulator to drive a boost converter, converting 5 volts to 70 volts with a single 22uh inductor. The Uniblitz driver circuit serves as the basis for a precision circuit for piezo tube control. The Pic16F628 also has on the chip a 16 bit counter, we plan to use this to eliminate the PCI-6036e allowing the device to operate from a single RS232 port. With this photon counting system we can explore the use of luminescent chemical sensors and weak florescent signals located in cells and in the boundary layers.