LOW LIGHT AND LUMINESCENT DETECTION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 作为创建综合实验平台的努力的一部分，将现有的和正在开发的技术结合在一起，我们的目标是在BRC中结合微光和发光成像。在发光方面，我们针对的是ATP的荧光素/荧光素酶测定。这种没有商业来源的成像仪的成功将对几个对新陈代谢问题感兴趣的用户项目产生立竿见影的影响。为了观察细胞群体，我们还试图开发一种更简单但有效的光子计数系统。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As part of the drive to create integrated experimental platforms bringing together established and developing techniques we made it a goal to incorporate low light and luminescent imaging within the BRC. For luminescence we are targeting the luciferin/luciferase assay for ATP. Success with this imager, for which there are no commercial sources, will have an immediate impact on several user projects interested in matters of metabolism. For looking at populations of cells we have also sought to develop a simpler but effective photon counting system.