IMPROVEMENT, UPGRADING, RESTRUCTURING OF EXISTING LFD INSTRUMENTATION

现有 LFD 仪器的改进、升级、重组

• 批准号: 7956550
• 负责人: ENRICO GRATTON
• 金额: $ 8.02万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956550
• 关键词: Amplifiers; Computer Retrieval of Information on Scientific Projects Database; Computer software; Detection; Electronics; Fluorescence; Funding; General Population; Grant; Institution; Laboratories; Measurement; Microscope; Modification; Research; Research Personnel; Resources; Source; Time; United States National Institutes of Health; base; data acquisition; instrument; instrumentation; user-friendly

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. All previously existing five microscope setups of the LFD have to be subjected to instrumental upgrading. Discriminators have to be displaced by constant fraction discriminators and new pre-amplifiers have to be installed on all instruments. These modifications are the prerequisite for the replacement of the detection electronics on all instruments. In addition to the microscope setups, various cuvette-based fluorometers for time-resolved fluorescence measurements await upgrading or will be newly assembled. All fluorometers shall be controlled by the new and user-friendly Windows-based control software of the company ISS in addition to optional control via the software package SimFCS, which is made available to the general public by our laboratory and used for instrument control and data acquisition and analysis of all microscope setups.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 LFD所有现有的五个显微镜装置都必须进行仪器升级。必须用恒定分数鉴别器取代鉴别器，并且必须在所有仪器上安装新的前置放大器。这些修改是更换所有仪器上的检测电子设备的先决条件。 除了显微镜装置外，用于时间分辨荧光测量的各种基于试管的荧光计正在等待升级或将被新组装。除了通过SimFCS软件包进行可选控制外，所有荧光计都应由ISS公司基于Windows的新的、用户友好的控制软件控制，该软件包由我们的实验室向公众提供，用于所有显微镜设置的仪器控制、数据采集和分析。