IN VIVO ASSESSMENT OF INTERVERTEBRAL DISC COLLAGEN CONTENT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 磁化转移率已被证明与生物中的胶原含量有很好的相关性 组织。先前的研究表明，椎间盘的核区域包含 明显聚集随机定向的II型胶原纤维。然而，原子核 胶原蛋白含量对年龄相关性退变的反应还没有得到彻底的研究， 而蛋白多糖的含量已显示出随着退化而持续下降。在……里面 本研究，我们将利用年轻人和老年人的磁化传递比图来调查 退变由于退变而导致的盘核中胶原含量的变化。为了 将直接饱和效应降至最低，我们将在非共振频率施加饱和脉冲 6400赫兹。成像将在西门子1.5T索纳塔磁共振扫描仪上进行。一种习俗-- 将使用带有离共振饱和选项的编程TSE脉冲序列来采集 计算磁化传递比图所需的图像。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Magnetization transfer ratio has been shown to correlate well with collagen content in biological tissue. Previous studies have shown that the nucleus region of intervertebral disc contains significant concentration of randomly oriented type II collagen fibers. However, the nucleus collagen content's response to age-related degeneration has not been thoroughly investigated, while the proteoglycan content has been shown to consistently decrease with degeneration. In this study, we will use magnetization transfer ratio maps of young and old subjects to investigate the change in collagen content occurring in disc nucleus as a result of degeneration. In order to minimize direct saturation effect, we will apply the saturation pulse at an off-resonance frequency of 6400 Hz. Imaging will be carried out on a Siemens 1.5T Sonata MRI scanner. A custom- programmed TSE pulse sequence with the option of off-resonance saturation will be used to collect the images required to compute magnetization transfer ratio maps.