IN VIVO ASSESSMENT OF INTERVERTEBRAL DISC COLLAGEN CONTENT

椎间盘胶原含量的体内评估

• 批准号: 8169058
• 负责人: CHENYANG WANG
• 金额: $ 0.87万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169058
• 关键词: Biological; Cell Nucleus; Clinical assessments; Collagen; Collagen Fiber; Computer Retrieval of Information on Scientific Projects Database; Custom; Frequencies; Funding; Grant; Image; Institution; Intervertebral disc structure; Magnetic Resonance Imaging; Maps; Physiologic pulse; Proteoglycan; Research; Research Personnel; Resources; Source; Tissues; United States National Institutes of Health; Weight; Zaleplon; age related; in vivo; intervertebral disk degeneration; programs; radiologist; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Magnetization transfer ratio has been shown to correlate well with collagen content in biological tissue. Previous studies have shown that the nucleus region of intervertebral disc contains significant concentration of randomly oriented type 11 collagen fibers. However, the nucleus collagen content's response to age-related degeneration has not been thoroughly investigated, while the proteoglycan content has been shown to consistently decrease with degeneration. In this study, we will use magnetization transfer ratio maps of young and old subjects to investigate the change in collagen content occurring in disc nucleus as a result of degeneration. The degree of interverbral disc degeneration will be independently assessed via T2-weighted MR images according to the Pfirrmann scale. Grading as well as clinical assessment of the intervertebral discs will be carried out by a board-certified radiologist. Correlation analysis between magnetization transfer ratio and Pfirrmann grade will elucidate the fate of nucleus collagen content in intervertebral disc degeneration. In order to minimize direct saturation effect, we will apply the saturation pulse at an off-resonance frequency of 6.4 kHz. Imaging will be carried out on a Siemens 1.5T Sonata MRI scanner. A custom programmed centrically-encoded TSE pulse sequence with the option of off-resonance saturation will be used to collect the images required to compute magnetization transfer ratio maps.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 磁化传递率已被证明与生物样品中的胶原蛋白含量有很好的相关性。 组织.以往的研究表明，椎间盘的核区含有 随机取向的11型胶原纤维的显著浓度。然而，原子核 胶原蛋白含量对年龄相关性退化的反应尚未被彻底研究， 而蛋白聚糖含量已显示随着变性而一致地降低。在 在本研究中，我们将使用年轻和老年受试者的磁化转移率图来调查 退变导致椎间盘髓核中胶原含量的变化。程度 将通过T2加权MR图像独立评估椎间盘退变 根据Pfirrmann量表。椎间盘的分级和临床评估 将由一名经过认证的放射科医生进行磁化强度与 转移率和Pfirrmann分级将阐明椎间融合器中细胞核胶原含量的命运。 椎间盘退变为了最小化直接饱和效应，我们将在 非共振频率为6.4 kHz。将在Siemens 1.5T Sonata MRI上进行成像 扫描仪自定义编程的中央编码TSE脉冲序列， 非共振饱和将用于收集计算磁化所需的图像 传输比率图。