IN VIVO ASSESSMENT OF INTERVERTEBRAL DISC COLLAGEN CONTENT

椎间盘胶原含量的体内评估

• 批准号: 8361969
• 负责人: CHENYANG WANG
• 金额: $ 3.84万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361969
• 关键词: Biological; Cell Nucleus; Clinical assessments; Collagen; Collagen Fiber; Custom; Frequencies; Funding; Grant; Image; Intervertebral disc structure; Magnetic Resonance; Magnetic Resonance Imaging; Maps; National Center for Research Resources; Physiologic pulse; Principal Investigator; Proteoglycan; Research; Research Infrastructure; Resources; Source; Tissues; United States National Institutes of Health; Weight; Zaleplon; age related; cost; in vivo; intervertebral disk degeneration; optical imaging; programs; radiologist; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Magnetization transfer ratio has been shown to correlate well with collagen content in biological tissue. Previous studies have shown that the nucleus region of intervertebral disc contains significant concentration of randomly oriented type 11 collagen fibers. However, the nucleus collagen content's response to age-related degeneration has not been thoroughly investigated, while the proteoglycan content has been shown to consistently decrease with degeneration. In this study, we will use magnetization transfer ratio maps of young and old subjects to investigate the change in collagen content occurring in disc nucleus as a result of degeneration. The degree of interverbral disc degeneration will be independently assessed via T2-weighted MR images according to the Pfirrmann scale. Grading as well as clinical assessment of the intervertebral discs will be carried out by a board-certified radiologist. Correlation analysis between magnetization transfer ratio and Pfirrmann grade will elucidate the fate of nucleus collagen content in intervertebral disc degeneration. In order to minimize direct saturation effect, we will apply the saturation pulse at an off-resonance frequency of 6.4 kHz. Imaging will be carried out on a Siemens 1.5T Sonata MRI scanner. A custom programmed centrically-encoded TSE pulse sequence with the option of off-resonance saturation will be used to collect the images required to compute magnetization transfer ratio maps.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 磁化转移率已被证明与生物中的胶原含量有很好的相关性 组织。先前的研究表明，椎间盘的核区域包含 明显聚集随机定向的11型胶原纤维。然而，原子核 胶原蛋白含量对年龄相关性退变的反应还没有得到彻底的研究， 而蛋白多糖的含量已显示出随着退化而持续下降。在……里面 本研究，我们将利用年轻人和老年人的磁化传递比图来调查 退变由于退变而导致的盘核中胶原含量的变化。学位 将通过T2加权MR图像独立地评估椎间盘退变 根据普菲尔曼分级标准。腰椎间盘的分级及临床评价 将由委员会认证的放射科医生进行。磁化强度之间的相关分析 转移率和Pfirmann分级将阐明髓核内胶原含量的去向 椎间盘退变。为了将直接饱和效应降至最低，我们将在 偏离共振频率为6.4千赫。成像将在西门子1.5T索纳塔磁共振上进行 扫描仪。具有以下选项的定制编程中心编码TSE脉冲序列 将使用非共振饱和来收集计算磁化所需的图像 传输比贴图。