SALIVARY GLAND EXOSOME PROTEOME

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 唾液是一种富含超过一千种独特蛋白质的口腔液体，这些蛋白质通过几种途径分泌，可能包括外泌体。外泌体是组成性分泌的多泡内体内的内体来源的膜结合囊泡。采用基于质谱的鸟枪蛋白质组学方法来探索唾液中发现的许多蛋白质来源于外泌体的可能性。使用MudPIT（多维蛋白质鉴定技术），我们对人腮腺唾液外泌体组分中的491种蛋白质进行了编目。腮腺分泌外泌体，其可携带可用于口腔和全身性疾病的诊断、预后和治疗的信息蛋白质标记物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Saliva is an oral fluid rich in over a thousand unique proteins which are secreted by several pathways, possibly including exosomes. Exosomes are membrane-bound vesicles of endosomal origin within multivesicular endosomes that are constitutively secreted. A mass spectrometry-based shotgun proteomics approach was employed to explore the possibility that many of the proteins found in saliva are derived from exosomes. Using MudPIT (multidimensional protein identification technology), we catalogued 491 proteins in the exosome fraction of human parotid saliva. Parotid glands secrete exosomes that may carry informative protein markers useful in the diagnosis, prognosis and treatment of oral and systemic diseases.