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PROTEOMIC ANALYSIS OF ZYGOTE AND OOKINETE STAGES OF THE AVIAN MALARIA PARASITE P

PROTEOMIC ANALYSIS OF ZYGOTE AND OOKINETE STAGES OF THE AVIAN MALARIA PARASITE P
禽疟原虫 P 受精卵和动合子阶段的蛋白质组学分析
批准号:
7957733
负责人:
JOHN L YATES
金额:
$0.33万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
已结题
起止时间:
2009-09-01 至 2010-08-31

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中文摘要
翻译
这个子项目是众多研究子项目之一
英文摘要
This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Delineation of the complement of proteins comprising the zygote and ookinete, the early developmental stages of Plasmodium within the mosquito midgut, is fundamental to understand initial molecular parasite-vector interactions. The published proteome of Plasmodium falciparum does not include analysis of the zygote/ookinete stages, nor does that of P. berghei include the zygote stage or secreted proteins. P. gallinaceum zygote, ookinete, and ookinete-secreted/released protein samples were prepared and subjected to Multidimensional protein identification technology (MudPIT). Peptides of P. gallinaceum zygote, ookinete, and ookinete-secreted proteins were identified by MS/MS, mapped to ORFs (> 50 amino acids) in the extent P. gallinaceum whole genome sequence, and then matched to homologous ORFs in P. falciparum. A total of 966 P. falciparum ORFs encoding orthologous proteins were identified; just over 40% of these predicted proteins were found to be hypothetical. A majority of putative proteins with predicted secretory signal peptides or transmembrane domains were hypothetical proteins. This analysis provides a more comprehensive view of the hitherto unknown proteome of the early mosquito midgut stages of P. falciparum. The results underpin more robust study of Plasmodium-mosquito midgut interactions, fundamental to the development of novel strategies of blocking malaria transmission.
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INVESTIGATION OF THE POROUS LAYER OPEN TUBULAR (PLOT) REVERSE PHASE COLUMN
  • 批准号:
    7957760
  • 项目类别:
  • 资助金额:
    $2.09万
  • 财政年份:
    2009
  • 负责人:
    JOHN L YATES
  • 依托单位:
A HUPO TEST SAMPLE STUDY REVEALS COMMON PROBLEMS IN MASS SPECTROMETRY-BASED PROT
  • 批准号:
    7957726
  • 项目类别:
  • 资助金额:
    $0.33万
  • 财政年份:
    2009
  • 负责人:
    JOHN L YATES
  • 依托单位:
GENOME ANNOTATION BY MASS SPECTROMETRY
  • 批准号:
    7957688
  • 项目类别:
  • 资助金额:
    $0.33万
  • 财政年份:
    2009
  • 负责人:
    JOHN L YATES
  • 依托单位:
IDENTIFYING DIFFERENCES IN PROTEIN EXPRESSION LEVELS BY SPECTRAL COUNTING AND FE
  • 批准号:
    7957728
  • 项目类别:
  • 资助金额:
    $2.09万
  • 财政年份:
    2009
  • 负责人:
    JOHN L YATES
  • 依托单位:
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