INVESTIGATION OF THE POROUS LAYER OPEN TUBULAR (PLOT) REVERSE PHASE COLUMN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 多孔层开管(PLOT)色谱柱的灵敏度和保留重现性优于固定床固相反相色谱柱。10um身份证。当用于生物分子分离时，聚(二乙烯基苯-苯乙烯)柱允许比典型的填充柱有更高的扩散速率，这改善了峰形和色谱重现性。本研究旨在评估聚(二乙烯基苯-苯乙烯)PLOT色谱柱的载气能力和稳定性，并与传统的固定相C-18RP色谱柱进行比较。总体目标是在MudPIT设置中使用绘图列进行高复杂性样本分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Porous Layer Open Tubular (PLOT) chromatographic columns have been reported to have characteristics such as sensitivity and retention reproducibility superior to that of the packed bed solid phase RP columns. The 10um i.d. poly-(divinylbenzene-styrene) columns allow for higher diffusion rates than a typical packed column when used in biological molecule separation, which improves peak shape and chromatographic reproducibility. The present study is aimed at estimating such parameters loading capacity and robustness of the poly-(divinyl-benzene-styrene) PLOT columns and their comparison to traditional packed solid phase C-18 RP columns. The overall goal is to use PLOT columns in MudPIT setup for high complexity sample analysis.