INVESTIGATION OF THE POROUS LAYER OPEN TUBULAR (PLOT) REVERSE PHASE COLUMN

多孔层开管（小区）反相柱的研究

• 批准号: 7957760
• 负责人: JOHN L YATES
• 金额: $ 2.09万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957760
• 关键词: 1,3-Butadiene; Beds; Benzene; Biological; Biology; Characteristics; Computer Retrieval of Information on Scientific Projects Database; Diffusion; Funding; Fungal Genome; Goals; Grant; Institution; Investigation; Phase; Reporting; Reproducibility; Research; Research Personnel; Resources; Sampling; Shapes; Solid; Source; Tubular formation; United States National Institutes of Health; improved

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Porous Layer Open Tubular (PLOT) chromatographic columns have been reported to have characteristics such as sensitivity and retention reproducibility superior to that of the packed bed solid phase RP columns. The 10um i.d. poly-(divinylbenzene-styrene) columns allow for higher diffusion rates than a typical packed column when used in biological molecule separation, which improves peak shape and chromatographic reproducibility. The present study is aimed at estimating such parameters loading capacity and robustness of the poly-(divinyl-benzene-styrene) PLOT columns and their comparison to traditional packed solid phase C-18 RP columns. The overall goal is to use PLOT columns in MudPIT setup for high complexity sample analysis.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 据报道，多孔层开管 (PLOT) 色谱柱的灵敏度和保留重现性等特性优于填充床固相反相色谱柱。 10um 内径当用于生物分子分离时，聚（二乙烯基苯-苯乙烯）柱比典型的填充柱具有更高的扩散速率，从而改善峰形和色谱重现性。 本研究旨在评估聚（二乙烯基苯苯乙烯）PLOT 柱的负载能力和稳健性等参数，并将其与传统填充固相 C-18 RP 柱进行比较。 总体目标是在 MudPIT 设置中使用 PLOT 柱进行高复杂性样品分析。