INVESTIGATION OF THE POROUS LAYER OPEN TUBULAR (PLOT) REVERSE PHASE COLUMN

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 多孔层开管（PLOT）色谱柱具有上级填充床固相反相色谱柱的灵敏度和保留重现性等特性。 10um I.D.当用于生物分子分离时，聚-（二乙烯基苯-苯乙烯）柱允许比典型的填充柱更高的扩散速率，这改善了峰形和色谱再现性。 本研究的目的是估计这些参数的负载能力和耐用性的聚-（二乙烯基-苯-苯乙烯）PLOT柱和传统的填充固相C-18反相柱的比较。 总体目标是在MudPIT设置中使用PLOT色谱柱进行高复杂性样品分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Porous Layer Open Tubular (PLOT) chromatographic columns have been reported to have characteristics such as sensitivity and retention reproducibility superior to that of the packed bed solid phase RP columns. The 10um i.d. poly-(divinylbenzene-styrene) columns allow for higher diffusion rates than a typical packed column when used in biological molecule separation, which improves peak shape and chromatographic reproducibility. The present study is aimed at estimating such parameters loading capacity and robustness of the poly-(divinyl-benzene-styrene) PLOT columns and their comparison to traditional packed solid phase C-18 RP columns. The overall goal is to use PLOT columns in MudPIT setup for high complexity sample analysis.