EVALUATION OF LTQ-ORBITRAP
EVALUATION OF LTQ-ORBITRAP
批准号:
7957739
负责人:
JOHN L YATES
金额:
$2.09万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
已结题
起止时间:
2009-09-01 至 2010-08-31
关键词:
AmericanBiologyComplex MixturesComputer Retrieval of Information on Scientific Projects DatabaseDataData SetDatabasesEvaluationFundingFungal GenomeGrantHybridsInstitutionIonsMass Spectrum AnalysisNoisePeptidesPerformanceProteinsPublishingResearchResearch PersonnelResourcesScanningSignal TransductionSocietiesSoftware ValidationSourceSpeedUnited States National Institutes of Healthimprovedinstrumentmass spectrometer
中文摘要
这个子项目是许多研究子项目中利用
资源由NIH/NCRR资助的中心拨款提供。子项目和
调查员(PI)可能从NIH的另一个来源获得了主要资金,
并因此可以在其他清晰的条目中表示。列出的机构是
该中心不一定是调查人员的机构。
我们评估了用于数据依赖采集的离子丰度阈值设置对
一种混合LTQ-Orbitrap质谱仪,分析了
收集的光谱,全MS扫描的信噪比,光谱质量
获得串联质谱图,以及从一种植物中鉴定的多肽和蛋白质的数量
复杂的混合物。我们发现,提高数据依赖获取的门槛
通常会减少获取光谱的数量,但会提高获取的光谱的质量。这是
当阈值设置高于完全MS扫描的噪声级别时,尤其如此。我们
比较两种不同的实验配置:一种是在
Orbitrap分析器,而串联MS扫描是在LTQ分析器中获取的,其中
全MS和串联MS扫描均在LTQ分析仪中采集。我们检查了这个数字
在不同的阈值条件下鉴定出的光谱、多肽和蛋白质,我们发现
最佳阈值设置等于或低于仪器各自的噪声水平
无论是在Orbitrap还是在LTQ分析仪中执行全MS扫描。
在比较两种分析仪的高通量识别性能时,我们
结论是,在最佳阈值水平下,LTQ和Orbitrap识别出类似的
多肽和蛋白质的数量。LTQ的扫描速度越高,会产生更多
被收集的光谱,大致被轨道诱捕器更高的质量精度所补偿,
这带来了改进的数据库搜索和多肽验证软件
性能。(J am Soc MS Spectrm 2009,xx,xxx)?2009由Elsevier Inc.于
代表美国质谱学学会
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
We evaluate the effect of ion-abundance threshold settings for data-dependent acquisition on
a hybrid LTQ-Orbitrap mass spectrometer, analyzing features such as the total number of
spectra collected, the signal to noise ratio of the full MS scans, the spectral quality of the
tandem mass spectra acquired, and the number of peptides and proteins identified from a
complex mixture. We find that increasing the threshold for data-dependent acquisition
generally decreases the quantity but increases the quality of the spectra acquired. This is
especially true when the threshold setting is set above the noise level of the full MS scan. We
compare two distinct experimental configurations: one where full MS scans are acquired in the
Orbitrap analyzer while tandem MS scans are acquired in the LTQ analyzer, and one where
both full MS and tandem MS scans are acquired in the LTQ analyzer. We examine the number
of spectra, peptides, and proteins identified under various threshold conditions, and we find
that the optimal threshold setting is at or below the respective noise level of the instrument
regardless of whether the full MS scan is performed in the Orbitrap or in the LTQ analyzer.
When comparing the high-throughput identification performance of the two analyzers, we
conclude that, used at optimal threshold levels, the LTQ and the Orbitrap identify similar
numbers of peptides and proteins. The higher scan speed of the LTQ, which results in more
spectra being collected, is roughly compensated by the higher mass accuracy of the Orbitrap,
which results in improved database searching and peptide validation software
performance. (J Am Soc Mass Spectrom 2009, xx, xxx) ? 2009 Published by Elsevier Inc. on
behalf of American Society for Mass Spectrometry
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
INVESTIGATION OF THE POROUS LAYER OPEN TUBULAR (PLOT) REVERSE PHASE COLUMN
-
批准号:7957760
-
项目类别:
-
资助金额:$2.09万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
A HUPO TEST SAMPLE STUDY REVEALS COMMON PROBLEMS IN MASS SPECTROMETRY-BASED PROT
-
批准号:7957726
-
项目类别:
-
资助金额:$0.33万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
GENOME ANNOTATION BY MASS SPECTROMETRY
-
批准号:7957688
-
项目类别:
-
资助金额:$0.33万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
IDENTIFYING DIFFERENCES IN PROTEIN EXPRESSION LEVELS BY SPECTRAL COUNTING AND FE
-
批准号:7957728
-
项目类别:
-
资助金额:$2.09万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
CUSTOM DATA ACQUISITION USING THERMOELECTRON CONTROL OBJECT MODEL (COM) LIBRARY
-
批准号:7957761
-
项目类别:
-
资助金额:$2.09万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
LABEL-FREE QUANTIFICATION VIA IMPROVED CHROMATOGRAPHIC ELUTION REPRODUCIBILITY
-
批准号:7957762
-
项目类别:
-
资助金额:$2.09万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
COLANDER: A PROBABILITY-BASED SUPPORT VECTOR MACHINE ALGORITHM FOR AUTOMATIC SCR
-
批准号:7957740
-
项目类别:
-
资助金额:$2.09万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
ANALYSIS OF ORGANELLES BY ON-LINE TWO-DIMENSIONAL LIQUID CHROMATOGRAPHY-TANDEM M
-
批准号:7957738
-
项目类别:
-
资助金额:$0.33万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
SEMINARS GIVEN BY JOHN R YATES III
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批准号:7957693
-
项目类别:
-
资助金额:$1.3万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
ETD CHARGE PREDICTION MACHINE
-
批准号:7957731
-
项目类别:
-
资助金额:$2.09万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
CAPILLARY ELECTROPHORESIS - MASS SPECTROMETRY ON PROTEOMIC ANALYSIS
-
批准号:7957723
-
项目类别:
-
资助金额:$2.09万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
MOTIF-SPECIFIC SAMPLING OF PHOSPHOPROTEOMES
-
批准号:7957767
-
项目类别:
-
资助金额:$2.09万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
COMPARISONS OF MASS SPECTROMETRY COMPATIBLE SURFACTANTS FOR GLOBAL ANALYSIS OF T
-
批准号:7957724
-
项目类别:
-
资助金额:$0.33万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
IMPROVING PROTEIN IDENTIFICATION SENSITIVITY BY COMBINING MS AND MS/MS
-
批准号:7957770
-
项目类别:
-
资助金额:$2.09万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
PROTEOMIC ANALYSIS OF ZYGOTE AND OOKINETE STAGES OF THE AVIAN MALARIA PARASITE P
-
批准号:7957733
-
项目类别:
-
资助金额:$0.33万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
SALIVARY GLAND EXOSOME PROTEOME
-
批准号:7957758
-
项目类别:
-
资助金额:$0.33万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
MASS SPECTROMETRY FOR PROTEOMICS
-
批准号:7957667
-
项目类别:
-
资助金额:$0.33万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
PATTERNLAB FOR PROTEOMICS: A TOOL FOR DIFFERENTIAL SHOTGUN PROTEOMICS
-
批准号:7957737
-
项目类别:
-
资助金额:$2.09万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
METHOD DEVELOPMENT FOR MEMBRANE PROTEIN ANALYSIS
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批准号:7957769
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项目类别:
-
资助金额:$2.09万
-
财政年份:2009
-
负责人:JOHN L YATES
-
依托单位:
FUNCTIONS OF EPSTEIN-BARR VIRUS NUCLEAR ANTIGEN 1
-
批准号:2091089
-
项目类别:
-
资助金额:$18.11万
-
财政年份:1986
-
负责人:JOHN L YATES
-
依托单位:
国内基金
海外基金
Journal of Integrative Plant Biology
-
批准号:31024801
-
项目类别:专项基金项目
-
资助金额:24.0万元
-
批准年份:2010
-
负责人:贺萍
-
依托单位: