IDENTIFICATION OF PROTEINS THAT REGULATE THE SIN3A HISTONE DEACETYLASE COMPLEX

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 组蛋白翻译后修饰在基因调控、染色质包装和细胞分化中起着关键作用。 组蛋白去乙酰化酶（HDAC）mSin 3A复合物参与转录抑制，并含有与生长控制有关的多个亚基，包括HDAC 1和2、乳腺转移抑制蛋白1（BRMS 1和BRMS 1 L1）和p33/ING 1肿瘤抑制蛋白。 然而，我们的初步数据表明，mSin 3A复合物在不同的细胞类型和不同的生长条件下可能是组成多样的。 不同Sin 3A亚基的分离和表征对于阐明各种mSin 3A复合物的作用是必不可少的。 使用质谱，我们的目标是识别相关的蛋白质，并确定它们如何与其他亚基相互作用。 这些数据将指导功能研究，以确定对细胞增殖，基因表达和基因组稳定性的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Histone post-translational modifications play a pivotal role in gene regulation, chromatin packaging and cellular differentiation. The histone deacetylase (HDACs) mSin3A complex is involved in transcriptional repression and contains multiple subunits that have been implicated in growth control, including HDAC 1 and 2, the Breast Suppressor of Metastasis 1 proteins (BRMS1 and BRMS1L1), and the p33/ING1 tumor suppressor proteins. However, our preliminary data suggest that mSin3A complex may be compositionally diverse in different cell types and under different growth conditions. Isolation and characterization of different Sin3A subunits is essential for elucidating the role of the various mSin3A complexes. Using mass spectroscopy, we aim to identify associated proteins, and determine how they interact with other subunits. These data will guide functional studies to determine effects on cell proliferation, gene expression and genomic stability.