SAXS CHARACTERIZATION OF HELICAL SYNTHETIC POLYPEPTIDES

螺旋合成多肽的 SAXS 表征

• 批准号: 7954919
• 负责人: FREDERICK L BEYER
• 金额: $ 0.65万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954919
• 关键词: Affect; Binding; Biological; Biology; Biophysics; Chemical Structure; Cholera; Computer Retrieval of Information on Scientific Projects Database; Data; Event; Funding; Grant; Inflammation; Institution; Medical; Neoplasm Metastasis; Polymers; Proteins; Research; Research Personnel; Resources; Saline; Shapes; Source; Structure; Synthesis Chemistry; Therapeutic; Toxin; United States National Institutes of Health; design; polypeptide; research study; sugar; synthetic protein

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our project, entitled "SAXS Characterization of Helical Synthetic Polypeptides," seeks to understand the shape of our unusual molecules when they are dissolved in a saline solution. These new molecules, where are made from the same building blocks as proteins, were designed to explore the effect of molecule shape and structure on the way proteins interact with toxins like Cholera. The manner in which proteins interact with saccharides (sugars) is critical in many important events in biology, such as inflammation, metastasis, and toxin binding. Although synthetic polymers can be useful in affecting these kinds of events, it is difficult to make synthetic polymers with enough control to clearly understand the polymer/sugar interactions that are important. We think that specific chemical structures, and how the molecules "display" them to each other, are important in the biological events described above. Our approach is to use a combination of synthetic chemistry and biological synthesis to make synthetic proteins that include these very specific chemical structures. Equally importantly, our approach should allow us to control how our molecules "display" those chemical structures because it allows us to control what the shapes of the molecules are when they are dissolved in saline. Our preliminary results from SAXS experiments conducted at the APS indicate that our molecules have the shapes we anticipated. Knowing the shapes of our molecules will allow us to interpret our data regarding the way these molecules interact with saccharides, and allow us to create new materials for medical and therapeutic applications.

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们的项目名为“螺旋合成多肽的SAXS表征”，旨在了解我们不寻常的分子溶解在盐溶液中时的形状。 这些新分子是由与蛋白质相同的结构单元制成的，旨在探索分子形状和结构对蛋白质与霍乱等毒素相互作用方式的影响。 蛋白质与糖相互作用的方式在生物学中的许多重要事件中至关重要，例如炎症，转移和毒素结合。 虽然合成聚合物可以用于影响这些类型的事件，但很难以足够的控制来制造合成聚合物以清楚地理解重要的聚合物/糖相互作用。 我们认为，特定的化学结构，以及分子如何相互“展示”它们，在上述生物事件中很重要。 我们的方法是使用合成化学和生物合成的组合来制造包含这些非常特定的化学结构的合成蛋白质。 同样重要的是，我们的方法应该允许我们控制我们的分子如何“显示”这些化学结构，因为它允许我们控制分子溶解在盐水中时的形状。 我们在APS进行的SAXS实验的初步结果表明，我们的分子具有我们预期的形状。 了解我们分子的形状将使我们能够解释有关这些分子与生物素相互作用方式的数据，并使我们能够为医疗和治疗应用创造新材料。