DNA Sequencing
DNA测序
基本信息
- 批准号:7685023
- 负责人:
- 金额:$ 27.27万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-06-15 至 2014-05-31
- 项目状态:已结题
- 来源:
- 关键词:Acquired Immunodeficiency SyndromeArtsAutomationBackBase PairingBase SequenceBasic ScienceBioinformaticsBiologicalBiological AssayBudgetsCapillary ElectrophoresisChargeCollaborationsComplexComputer AnalysisComputer softwareConsultationsContractsDNADNA SequenceDNA Sequence AnalysisDNA Sequencing FacilityDatabasesDetectionDevelopmentEnsureGenesGenetic RecombinationGenetic VariationGenomeGenomicsGrantHIVImmune responseIn VitroIndividualLaboratoriesLengthLicensingMaintenanceManagement Information SystemsMeasuresMethodologyMethodsMicrosatellite RepeatsModemsMolecularMolecular BiologyMolecular GeneticsMonitorOnline SystemsOperative Surgical ProceduresPathogenesisPerformancePopulationPreparationProcessProtocols documentationQuality ControlReactionReagentResearchResearch PersonnelResearch Project GrantsResourcesSIVSamplingSequence AnalysisSequence DeterminationSeriesServicesSiteSystemTechniquesTechnologyTimeTrainingTraining SupportZambiaanalytical toolcomparativedesigndrug discoveryenv Genesexpression cloninggene functiongenetic analysisinvestigator traininglecturesmembermultidisciplinaryprogramsquality assurancerepositorysuccesstooltool developmentvaccine development
项目摘要
The DNA Sequencing and Analysis Core has been in operation for the past twenty-one years and has
provided CFAR members with state-of-the-art automated sequencing (DNA Sequencing Facility) and
computer analysis capabilities (Molecular and Genetic Bioinformatics Facility). Core functions have
included development of new methodologies for the automated sequencing process, training of
investigators in the use of sequence analysis tools, development of new molecular biology approaches to
characterize HIV/SIV genetic diversity, as well as supporting multidisciplinary and multi-institutional projects
and collaborations. In the last budget period, the DNA Sequencing Facility has supported 62 CFAR
investigators and provided essential services for over 100 AIDS related grants and contracts. 194 million
base pairs of primary sequence were determined, generating over $2,100,000 in user charge-backs.
Recently implemented automation has increased core efficiency and allowed to reduce user chargebacks
from $8 to $6 per sequencing reaction. During the same time period, the Molecular and Genetic
Bioinformatics Facility has actively supported over 30 CFAR investigators, and provided essential services
for 62 AIDS related grants and contracts. Specific Aims of the Core are:
1. To continue to provide automated DNA sequencing capabilities to CFAR members through the
availability and maintenance of dedicated Applied Biosystems DNA Sequencers with capillary
electrophoresis systems.
2. To establish new methods, technologies and reagents to assist investigators in the characterization
of HIV/SIV genetic diversity.
3. To provide and maintain a comprehensive set of modem bioinformatic databases and analytical
tools for the analysis of genetic information, including gene and genomic sequences.
4. To provide technical support and training in the use of these bioinformatic resources.
DNA测序和分析核心已经运行了21年,
为CFAR成员提供最先进的自动测序(DNA测序设施),
计算机分析能力(分子和遗传生物信息学设施)。核心职能有
包括开发自动测序过程的新方法,
研究人员在使用序列分析工具,开发新的分子生物学方法,
描述艾滋病毒/SIV遗传多样性的特征,并支持多学科和多机构项目
和合作。在上一个预算期间,DNA测序设施支持了62个
调查员,并为100多项与艾滋病有关的赠款和合同提供基本服务。194百万
确定了一级序列的碱基对,产生了超过2 100 000美元的用户退款。
最近实施的自动化提高了核心效率,并减少了用户计费
从8美元到6美元不等。在同一时期,分子和遗传
生物信息学设施积极支持了30多名CFAR研究人员,并提供了必要的服务
获得62项与艾滋病有关的赠款和合同。核心的具体目标是:
1.继续向CFAR成员提供自动DNA测序能力,
专用Applied Biosystems DNA测序仪的可用性和维护,
电泳系统
2.建立新的方法、技术和试剂,以协助研究者进行表征
艾滋病毒/SIV遗传多样性。
3.提供和维护一套全面的现代生物信息学数据库和分析
用于分析遗传信息的工具,包括基因和基因组序列。
4.在使用这些生物信息资源方面提供技术支持和培训。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Beatrice H Hahn其他文献
Beatrice H Hahn的其他文献
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{{ truncateString('Beatrice H Hahn', 18)}}的其他基金
Optimizing glycan shield coverage, germline B cell receptor binding and epitope diversity of V2-apex targeted HIV-1 Env immunogens
优化聚糖屏蔽覆盖、种系 B 细胞受体结合和 V2-apex 靶向 HIV-1 Env 免疫原的表位多样性
- 批准号:
10021396 - 财政年份:2019
- 资助金额:
$ 27.27万 - 项目类别:
Optimizing glycan shield coverage, germline B cell receptor binding and epitope diversity of V2-apex targeted HIV-1 Env immunogens
优化聚糖屏蔽覆盖、种系 B 细胞受体结合和 V2-apex 靶向 HIV-1 Env 免疫原的表位多样性
- 批准号:
10241429 - 财政年份:2019
- 资助金额:
$ 27.27万 - 项目类别:
Optimizing glycan shield coverage, germline B cell receptor binding and epitope diversity of V2-apex targeted HIV-1 Env immunogens
优化聚糖屏蔽覆盖、种系 B 细胞受体结合和 V2-apex 靶向 HIV-1 Env 免疫原的表位多样性
- 批准号:
10686018 - 财政年份:2019
- 资助金额:
$ 27.27万 - 项目类别:
Optimizing glycan shield coverage, germline B cell receptor binding and epitope diversity of V2-apex targeted HIV-1 Env immunogens
优化聚糖屏蔽覆盖、种系 B 细胞受体结合和 V2-apex 靶向 HIV-1 Env 免疫原的表位多样性
- 批准号:
10468221 - 财政年份:2019
- 资助金额:
$ 27.27万 - 项目类别:
Studies of the precursor of the human AIDS virus in its natural chimpanzee host
对黑猩猩天然宿主中人类艾滋病病毒前体的研究
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9186500 - 财政年份:2015
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8786805 - 财政年份:2014
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Restriction of HIV-1 transmission by type 1 interferons
1 型干扰素限制 HIV-1 传播
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9275913 - 财政年份:2014
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$ 27.27万 - 项目类别:
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