Molecular Biology of the Oocyte

卵泡发生：哺乳动物卵巢卵泡发生是一个复杂的过程，涉及生殖细胞和体细胞之间的相互作用。转录因子、细胞粘附分子和生长因子的精心表达是成功的必要条件。我们已经确定了一种生殖细胞特异性的，基本的螺旋-环-螺旋转录因子，FIGLA （factor In the GermLine, Alpha）。FIGLA在胚胎期13.5天（E13.5）首次被检测到，并在成年雌性小鼠中持续存在。最初被研究为雌性基因的激活剂，它也抑制雄性种系基因，以防止在卵子发生过程中不适当的表达。目前的研究已经确定了FIGLA的潜在卵母细胞特异性靶点，其重要作用如下：1)卵泡发生；2)施肥;3)植入前发育。

Folliculogenesis: Ovarian folliculogenesis in mammals is a complex process involving interactions between germ and somatic cells. Carefully orchestrated expression of transcription factors, cell adhesion molecules and growth factors are required for success. We have identified a germ-cell specific, basic helix-loop-helix transcription factor, FIGLA (Factor In the GermLine, Alpha). FIGLA is first detected at embryonic day 13.5 (E13.5) and persists in adult female mice. Initially investigated as an activator of female genes, it also represses male germline genes to prevent inappropriate expression during oogenesis. Current studies have identified potential oocyte-specific targets of FIGLA important for: 1) folliculogenesis; 2) fertilization; and 3) pre-implantation development. Fertilization: The specificity of sperm-egg recognition in mammals is mediated primarily by the zona pellucida. However, despite extensive investigation, there is no unifying model for the molecular basis of sperm interactions with the zona matrix that result in mammalian fertilization. Current studies investigate: 1) the molecular biology of the formation of the extracellular zona pellucida; 2) the molecular requirements of the zona pellucida matrix to support taxon-specific sperm-egg recognition; and 3) the mechanism by which post-fertilization polyspermy is prevented. Early Development: Activation of the embryonic genome in mice begins late in the one-cell zygote and is fully underway by the two-cell cleavage stage. Initial cell lineages that presage the inner cell mass and extra-embryonic trophectoderm are established when eight blastomeres compact to form polarized morulae in pre-implantation mouse development. In simpler model organisms, there is compelling evidence that persistent gene products from the egg are required for successful embryogenesis. However, in mice such effects have been documented only more recently and constitute a rapidly evolving area of investigation. Current studies investigate: 1) novel maternal effect genes that affect early mouse development; 2) components of a recently described maternally encoded subcortical complex; and 3) mechanisms by which the loss of the complex affects cleavage stage embryogenesis.