Maternal Effect Genes in Mammalian Embryogenesis

哺乳动物胚胎发生中的母体效应基因

• 批准号: 7593422
• 负责人: JURRIEN DEAN
• 金额: $ 57.5万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7593422
• 关键词: Affect; Animal Model; Antigens; Apical; Area; Binding; Biological Markers; Cell Lineage; Cell division; Cells; Complex; Development; Embryo; Embryonic Development; Fertilization; Genes; Genome; Germ Cells; Inner Cell Mass; Investigation; Localized; Morula; Mus; Nature; Oocytes; Play; Population; Proteins; Research Design; Role; Staging; Transcript; blastocyst; blastomere structure; egg; embryo cell; embryo stage 2; implantation; molecular asymmetry; novel; zygote

FILIA has been identified as a binding partner to MATER (Maternal Antigen That Embryos Require) which is encoded by a maternal effect gene. Products of each gene are detected in growing oocytes and, although transcripts are degraded prior to fertilization, the cognate proteins persist in early blastocysts. The two proteins co-localize to the cytocortex of ovulated eggs where the stability of FILIA is dependent on the presence of MATER. After fertilization, FILIA/MATER complexes become asymmetrically restricted in the apical cytocortex of two-cell embryos due to their absence in regions of cell-cell contact. This asymmetry is reversible upon disaggregation of blastomeres of the two- and four-cell embryo. Each protein persists in cells of the preimplantation embryo, but the continuous cell-cell contact of inner cells of the morulae seemingly precludes formation of the subcortical FILIA/MATER complex and results in cell populations that are marked by its presence (outer) or absence (inner). Thus, the FILIA/MATER complex provides a molecular marker of embryonic cell lineages, but it remains to be determined if the molecular asymmetry established after the first cell division plays a role in cell fate determinations in the early mouse embryo. If so, the plasticity of the FILIA/MATER complex localization may reflect the regulative nature of pre-implantation mouse development.

FILIA已被鉴定为母体效应基因编码的母体抗原（MATER）的结合伴侣。每个基因的产物都在生长中的卵母细胞中检测到，尽管转录本在受精前被降解，但同源蛋白在早期囊胚中持续存在。这两种蛋白共同定位于排卵卵子的细胞皮层，其中FILIA的稳定性依赖于MATER的存在。受精后，由于FILIA/MATER复合物在细胞-细胞接触区域的缺失，它们在双细胞胚胎的顶端细胞皮层受到不对称的限制。这种不对称在二细胞和四细胞胚胎的卵裂球解体后是可逆的。每种蛋白都存在于着床前胚胎的细胞中，但毛囊内细胞的连续细胞间接触似乎排除了皮层下FILIA/MATER复合物的形成，并导致细胞群体以其存在（外）或不存在（内）为标志。因此，FILIA/MATER复合体提供了胚胎细胞系的分子标记，但尚不清楚首次细胞分裂后建立的分子不对称是否在早期小鼠胚胎中决定细胞命运中起作用。如果是这样，FILIA/MATER复合物定位的可塑性可能反映了植入前小鼠发育的调节性质。