Molecular Biology of the Oocyte

卵母细胞的分子生物学

• 批准号: 8939496
• 负责人: JURRIEN DEAN
• 金额: $ 253.82万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8939496
• 关键词: Acrosome; Adult; Affect; Area; Binding; Biocompatible Materials; Cell Lineage; Cells; Childhood; Complement; Complex; Congenital Abnormality; Contraceptive methods; DNA; Development; Disease; Embryo; Embryonic Development; Ethics; Event; Exocytosis; Extracellular Matrix; Fertilization; Gene Components; Gene Transfer Techniques; Genes; Genetic Transcription; Genome; Germ Cells; Glycoproteins; Human; Human Development; Individual; Inner Cell Mass; Investigation; Mammalian Oviducts; Mammals; Mediating; Meiosis; Molecular; Molecular Biology; Morula; Mus; Oocytes; Oogenesis; Organelles; Ovary; Pathway interactions; Population Growth; Process; Proteins; Public Health; Reproductive Technology; Role; Specificity; Staging; Structure; Surface; Taxon; Zona Pellucida; base; egg; embryo cell; embryo stage 2; folliculogenesis; insight; macromolecule; mouse development; mouse model; novel; preimplantation; prevent; sperm cell; zygote

Maternal Effect Genes: Transcription that terminates during meiotic maturation of mouse eggs, resumes only after robust activation of the embryonic genome of the two-cell embryo. This interregnum between oocyte and embryonic gene transcription dictates a role for stored maternal factors. Encoded by maternal effect genes, these components accumulate during oogenesis and provide pathways and organelles essential for fertilization, activation of the embryonic genome and cleavage stage embryogenesis. Using mouse transgenesis, the role of individual or complexes of maternal factors is investigated in these processes. Fertilization: The taxon-specificity of sperm-egg recognition in mammals that results in fertilization is mediated primarily by the zona pellucida, an extracellular matrix surrounding ovulated eggs. Although a simple structure of 3-4 glycoproteins, the molecular basis of this sperm binding to the zona pellucida has been controversial. Current studies investigate: 1) molecular requirements to support taxon-specific sperm-egg recognition on the surface of the zona pellucida; 2) mechanism that result in acrosome exocytosis necessary for gamete fusion; and 3) the processes by which post-fertilization polyspermy is prevented. Early Development: Activation of the embryonic genome in mice begins late in the one-cell zygote and is fully underway by the two-cell cleavage stage. Initial cell lineages that presage the inner cell mass and extra-embryonic trophectoderm are established when eight blastomeres compact to form polarized morulae in pre-implantation mouse development. The role of maternal effect genes and organelles in these processes in mammals constitute a rapidly evolving area of inquiry. Current studies investigate: 1) novel maternal effect genes that affect early mouse development; 2) the role of maternal organelles in the maternal to zygotic transition; and 3) the role of degradation of maternal components in activation of the embryonic genome.

母性效应基因：在小鼠卵细胞减数分裂成熟过程中终止的转录，只有在两细胞胚胎的胚胎基因组被强烈激活后才恢复。卵母细胞和胚胎基因转录之间的空隙决定了储存的母体因子的作用。这些成分由母体效应基因编码，在卵子发生过程中积累，并为受精、胚胎基因组激活和卵裂期胚胎发生提供必要的途径和细胞器。使用小鼠转基因，在这些过程中，个别或复杂的母体因素的作用进行了研究。 施肥：哺乳动物精卵识别的分类特异性导致受精，主要是由透明质酸介导的，透明质酸是排卵卵周围的细胞外基质。虽然是3-4个糖蛋白的简单结构，但这种精子与透明体结合的分子基础一直存在争议。目前的研究调查：1）支持在透明壳表面上分类群特异性精卵识别的分子要求; 2）导致配子融合所必需的顶体胞吐作用的机制; 3）防止受精后多精受精的过程。 早期发育：小鼠胚胎基因组的激活开始于单细胞受精卵的晚期，并在双细胞分裂阶段完全进行。在着床前小鼠发育中，当八个卵裂球紧密形成极化桑椹胚时，就建立了预示内细胞团和胚外滋养外胚层的初始细胞谱系。母性效应基因和细胞器在哺乳动物这些过程中的作用构成了一个快速发展的调查领域。目前的研究调查：1）影响小鼠早期发育的新的母体效应基因; 2）母体细胞器在母体向合子转变中的作用; 3）母体成分的降解在胚胎基因组激活中的作用。